Size separation of sodium dodecyl sulfate complexes of human plasma proteins by capillary electrophoresis employing linear polyacrylamide as a sieving polymer

被引：27

作者：

Manabe, T ^{[1
]}

Oota, H ^{[1
]}

Mukai, J ^{[1
]}

机构：

[1] Ehime Univ, Fac Sci, Dept Chem, Matsuyama, Ehime 7900826, Japan

来源：

ELECTROPHORESIS | 1998年 / 19卷 / 13期

关键词：

sodium dodecyl sulfate electrophoresis; capillary electrophoresis; protein size separation; human plasma proteins; linear polyacrylamide;

D O I：

10.1002/elps.1150191310

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrophoretic conditions to separate sodium dodecyl sulfate (SDS) complexes of human plasma proteins according to their size differences, by capillary electrophoresis employing linear polyacrylamide as a sieving matrix (LPA-CE), have been examined. Using the optimized separation conditions,SDS complexes of human plasma proteins not treated with reducing agents were separated into about 40 peaks and shoulders within 60 min. The molecular glass values of major peaks in a separation pattern were estimated from a plot of molecular mass and migration time for standard proteins and some of the major plasma proteins have been identified on the pattern. The electrophoretic conditions were successfully applied for the analysis of proteins in immunoglobulin G (IgG) myeloma sera.

引用

页码：2308 / 2316

页数：9