Enzymatic recycling for signal amplification:: Improving microcystin detection with biosensors

被引：20

作者：

Campas, Monica ^{[1
]}

Olteanu, Maria G. ^{[1
]}

Marty, Jean-Louis ^{[1
]}

机构：

[1] Univ Perpignan, BIOMEM Grp, F-66860 Perpignan, France

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2008年 / 129卷 / 01期

关键词：

bioelectrocatalysis; substrate recycling; diaphorase; NADH oxidase; p-aminophenyl phosphate; electrochemical signal amplification;

D O I：

10.1016/j.snb.2007.08.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel strategy for the electrochemical signal amplification based on enzymatic recycling is presented. p-Aminophenyl phosphate was chosen as enzyme substrate and diaphorase and NADH oxidase as amplifying enzymes. Bioelectrocatalysis was observed by cyclic voltammetry using alkaline phosphatase as a model enzyme. Chronoamperometry experiments with diaphorase resulted in an amplification factor of 26.8. The amplification system was applied to the microcystin detection with a protein phosphatase 2A inhibition-based biosensor. The amplification enlarged the linear range by more than four orders of magnitude and decreased the limit of detection from 37.75 to 0.05 mu g/L, making the biosensor useful as a reliable screening tool to assess the quality of water. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：263 / 267

页数：5

共 42 条

[1] Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides [J].

Andreescu, S ;

Barthelmebs, L ;

Marty, JL .

ANALYTICA CHIMICA ACTA, 2002, 464 (02) :171-180

[2] AMPLIFIED ELECTROCHEMICAL IMMUNOASSAY FOR THYROTROPIN USING THERMOPHILIC BETA-NADH OXIDASE [J].

ATHEY, D ;

MCNEIL, CJ .

JOURNAL OF IMMUNOLOGICAL METHODS, 1994, 176 (02) :153-162

[3] Electrochemical detection of DNA hybridization based on silver-enhanced gold nanoparticle label [J].

Cai, H ;

Wang, YQ ;

He, PG ;

Fang, YH .

ANALYTICA CHIMICA ACTA, 2002, 469 (02) :165-172

[4] Towards the protein phosphatase-based biosensor for microcystin detection [J].

Campàs, M ;

Szydlowska, D ;

Trojanowicz, M ;

Marty, JL .

BIOSENSORS & BIOELECTRONICS, 2005, 20 (08) :1520-1530

[5] Enzyme inhibition-based biosensor for the electrochemical detection of microcystins in natural blooms of cyanobacteria [J].

Campas, Monica ;

Szydlowska, Dorota ;

Trojanowicz, Marek ;

Marty, Jean-Louis .

TALANTA, 2007, 72 (01) :179-186

[6] Amplification of amperometric biosensor responses by electrochemical substrate recycling.: 3.: Theoretical and experimental study of the phenol-polyphenol oxidase system immobilized in Laponite hydrogels and layer-by-layer self-assembled structures [J].

Coche-Guerente, L ;

Labbé, P ;

Mengeaud, V .

ANALYTICAL CHEMISTRY, 2001, 73 (14) :3206-3218

[7] Amplification of amperometric biosensor responses by electrochemical substrate recycling Part I.: Theoretical treatment of the catechol-polyphenol oxidase system [J].

Coche-Guérente, L ;

Desprez, V ;

Diard, JP ;

Labbé, P .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1999, 470 (01) :53-60

[8] Amplification of amperometric biosensor responses by electrochemical substrate recycling Part II.: Experimental study of the catechol-polyphenol oxidase system immobilized in a laponite clay matrix [J].

Coche-Guérente, L ;

Desprez, V ;

Labbé, P ;

Therias, S .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1999, 470 (01) :61-69

[9]

DELLACIANA L, 1995, J ELECTROANAL CHEM, V382, P129

[10] Electrochemical DNA sensors based on enzyme dendritic architectures:: An approach for enhanced sensitivity [J].

Domínguez, E ;

Rincón, O ;

Narváez, A .

ANALYTICAL CHEMISTRY, 2004, 76 (11) :3132-3138

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