Enzymatic recycling for signal amplification:: Improving microcystin detection with biosensors

被引：20

作者：

Campas, Monica ^{[1
]}

Olteanu, Maria G. ^{[1
]}

Marty, Jean-Louis ^{[1
]}

机构：

[1] Univ Perpignan, BIOMEM Grp, F-66860 Perpignan, France

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2008年 / 129卷 / 01期

关键词：

bioelectrocatalysis; substrate recycling; diaphorase; NADH oxidase; p-aminophenyl phosphate; electrochemical signal amplification;

D O I：

10.1016/j.snb.2007.08.009

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel strategy for the electrochemical signal amplification based on enzymatic recycling is presented. p-Aminophenyl phosphate was chosen as enzyme substrate and diaphorase and NADH oxidase as amplifying enzymes. Bioelectrocatalysis was observed by cyclic voltammetry using alkaline phosphatase as a model enzyme. Chronoamperometry experiments with diaphorase resulted in an amplification factor of 26.8. The amplification system was applied to the microcystin detection with a protein phosphatase 2A inhibition-based biosensor. The amplification enlarged the linear range by more than four orders of magnitude and decreased the limit of detection from 37.75 to 0.05 mu g/L, making the biosensor useful as a reliable screening tool to assess the quality of water. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：263 / 267

页数：5

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