β-phenylpyruvate and glucose uptake in isolated mouse soleus muscle and cultured C2C12 muscle cells

被引：7

作者：

Ben-Abraham, R

Gazit, V

Vofsi, O

Ben-Shlomo, I

Reznick, AZ

Katz, Y ^{[1
]}

机构：

[1] HaEmek Med Ctr, Dept Anesthesiol, IL-18101 Afula, Israel

[2] Tel Aviv Med Ctr & Sch Med, Dept Anesthesiol & Intens Care, IL-64239 Tel Aviv, Israel

[3] Technion Israel Inst Technol, Bruce Rappaport Fac Med, Lab Anesthesia Pain & Neural Res, IL-31096 Haifa, Israel

[4] HaEmek Med Ctr, Dept Obstet & Gynecol, IL-18101 Afula, Israel

[5] Technion Israel Inst Technol, Bruce Rappaport Fac Med, Dept Anat & Cell Biol, IL-31096 Haifa, Israel

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2003年 / 90卷 / 05期

关键词：

phenylpyruvic acid; glucose; skeletal muscle; cultured cells; phenylketonurias; glucose transporters;

D O I：

10.1002/jcb.10690

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous investigation demonstrated the potential of P-phenylpyruvate at high concentration to cause hypoglycemia in mice totally deprived of insulin. For further elucidation of the glucose-lowering mechanism, glucose uptake, and quantity of glucose transporters (GLUT1 and GLUT4) in mouse soleus muscle and C2C12 muscle cell lines were investigated following incubation with beta-phenylpyruvate in various concentrations. A marked enhancement of glucose uptake was demonstrated that peaked at 0.5 and 1.0 mM beta-phenylpyruvate in soleus muscle (P<0.01) and C2C12 cells (P<0.001), respectively. Kinetic analysis in C2C12 cells showed a twofold increase in V-max compared with controls (P<0.001). In addition, both GLUT1 and GLUT4 levels were increased following exposure to beta-phenylpyruvate. Our findings point to a peripheral hypoglycemic effect of beta-phenylpyruvate. (C) 2003 Wiley-Liss, Inc.

引用

页码：957 / 963

页数：7

共 24 条

[11] FACILITATIVE GLUCOSE TRANSPORTERS - REGULATORY MECHANISMS AND DYSREGULATION IN DIABETES [J].