Effects of phospholemman expression on swelling-activated ion currents and volume regulation in embryonic kidney cells

被引:23
作者
Davis, CE
Patel, MK
Miller, JR
John, JE
Jones, LR
Tucker, AL
Mounsey, JP
Moorman, JR
机构
[1] Univ Virginia, Hlth Sci Ctr, Dept Biomed Engn, Charlottesville, VA USA
[2] Univ Virginia, Hlth Sci Ctr, Dept Internal Med, Div Cardiovasc, Charlottesville, VA USA
[3] Univ Virginia, Hlth Sci Ctr, Dept Mol Physiol & Biol Phys, Charlottesville, VA USA
[4] Univ Virginia, Hlth Sci Ctr, Res Ctr, Dept Cardiovasc, Charlottesville, VA USA
[5] Indiana Univ, Sch Med, Krannert Inst Cardiol, Dept Internal Med, Indianapolis, IN 46202 USA
关键词
regulatory volume decrease; cell swelling; phospholemman; ion channel;
D O I
10.1023/B:NERE.0000010447.24128.ac
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Phospholemman (PLM) is a 72-amino-acid phosphoprotein that is a major substrate for cAMP-dependent protein kinase, protein kinase C, and NIMA kinase. In lipid bilayers, PLM forms ion channels selective for Cl-, K+, and taurine. Effluxes of these abundant intracellular osmolytes play an important role in the control of dynamic cell volume changes in many cell types. We measured swelling-activated ion currents and regulatory volume decrease (RVD) in human embryonic kidney cells stably overexpressing canine cardiac PLM. In response to swelling, two clonal cell lines overexpressing PLM had increased swelling-activated ion current densities and faster and more extensive RVD. A third clonal cell line overexpressing mutant PLM showed reduced ion current densities and a diminished RVD response. These results suggest a role for PLM in the regulation of cell volume, perhaps as a modulator of an endogenous swelling-activated signal transduction pathway or possibly by participating directly in swelling-induced osmolyte efflux.
引用
收藏
页码:177 / 187
页数:11
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