High-throughput SNP genotyping on universal bead arrays

被引：283

作者：

Shen, R ^{[1
]}

Fan, JB ^{[1
]}

Campbell, D ^{[1
]}

Chang, WH ^{[1
]}

Chen, J ^{[1
]}

Doucet, D ^{[1
]}

Yeakley, J ^{[1
]}

Bibikova, M ^{[1
]}

Garcia, EW ^{[1
]}

McBride, C ^{[1
]}

Steemers, F ^{[1
]}

Garcia, F ^{[1
]}

Kermani, BG ^{[1
]}

Gunderson, K ^{[1
]}

Oliphant, A ^{[1
]}

机构：

[1] Illumina Inc, San Diego, CA 92121 USA

来源：

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS | 2005年 / 573卷 / 1-2期

关键词：

SNP; genotyping; microarray; BeadArray;

D O I：

10.1016/j.mrfmmm.2004.07.022

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a flexible, accurate and highly multiplexed SNP genotyping assay for high-throughput genetic analysis of large populations on a bead array platform. The novel genotyping system combines high assay conversion rate and data quality with > 1500 multiplexing, and Array of Arrays (TM) formats. Genotyping assay oligos corresponding to specific SNP sequences are each linked to a unique sequence (address) that can hybridize to its complementary strand on universal arrays. The arrays are made of beads located in microwells of optical fiber bundles (Sentrix (R) Array Matrix) or silicon slides (Sentrix BeadChip). The optical fiber bundles are further organized into a matrix that matches a 96-well microtiter plate. The arrays on the silicon slides are multi-channel pipette compatible for loading multiple samples onto a single silicon slide. These formats allow many samples to be processed in parallel. This genotyping system enables investigators to generate approximately 300,000 genotypes per day with minimal equipment requirements and greater than 1.6 million genotypes per day in a robotics-assisted process. With a streamlined and comprehensive assay, this system brings a new level of flexibility, throughput, and affordability to genetic research. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：70 / 82

页数：13

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