A versatile assay for high-throughput gene expression profiling on universal array matrices

被引：137

作者：

Fan, JB ^{[1
]}

Yeakley, JM

Bibikova, M

Chudin, E

Wickham, E

Chen, J

Doucet, D

Rigault, P

Zhang, BH

Shen, R

McBride, C

Li, HR

Fu, XD

Oliphant, A

Barker, DL

Chee, MS

机构：

[1] Illumina Inc, San Diego, CA 92121 USA

[2] Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA

来源：

GENOME RESEARCH | 2004年 / 14卷 / 05期

关键词：

D O I：

10.1101/gr.2167504

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained using this assay for hormone-treated cell lines and laser-capture microdissected cancer tissues. Gene expression profiles derived from this assay were consistent with those determined by qRT-PCR. The DASL assay has been automated for use with a bead-based 96-array matrix system. The combined high-throughput assay and readout system is accurate and efficient, and can cost-effectively profile the expression of hundreds of genes in thousands of samples.

引用

收藏

页码：878 / 885

页数：8

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