The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia

被引：194

作者：

Schirenbeck, A

Bretschneider, T

Arasada, R

Schleicher, M

Faix, J

机构：

[1] Univ Munich, A Butenandt Inst Zellbiol, D-80336 Munich, Germany

[2] Max Planck Inst Biochem, AG Zelldynamik, D-82152 Martinsried, Germany

来源：

NATURE CELL BIOLOGY | 2005年 / 7卷 / 06期

关键词：

D O I：

10.1038/ncb1266

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Formins have important roles in the nucleation of actin and the formation of linear actin filaments, but their role in filopodium formation has remained elusive. Dictyostelium discoideum Diaphanous-related formin dDia2 is enriched at the tips of filopodia and interacts with profilin II and Rac1. An FH1FH2 fragment of dDia2 nucleated actin polymerization and removed capping protein from capped filament ends. Genetic studies showed that dDia2 is important for cell migration as well as the formation, elongation and maintenance of filopodia. Here we provide evidence that dDia2 specifically controls filopodial dynamics by regulating actin turnover at the barbed ends of actin filaments.

引用

收藏

页码：619 / U24

页数：8

相关论文

共 35 条

[1] Identification of a carboxyl-terminal diaphanous-related formin homology protein autoregulatory domain [J].

Alberts, AS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (04) :2824-2830

[2] Formin binding proteins bear WWP/WW domains that bind proline-rich peptides and functionally resemble SH3 domains [J].

Chan, DC ;

Bedford, MT ;

Leder, P .

EMBO JOURNAL, 1996, 15 (05) :1045-1054

[3] Membrane tether formation from blebbing cells [J].

Dai, JW ;

Sheetz, MP .

BIOPHYSICAL JOURNAL, 1999, 77 (06) :3363-3370

[4]

Dumontier M, 2000, J CELL SCI, V113, P2253

[5] CHARACTERIZATION OF ACTIN-BINDING AND LIPID-BINDING DOMAINS IN SEVERIN, A CA2+-DEPENDENT F-ACTIN FRAGMENTING PROTEIN [J].

EICHINGER, L ;

SCHLEICHER, M .

BIOCHEMISTRY, 1992, 31 (20) :4779-4787

[6] The genome of the social amoeba Dictyostelium discoideum [J].

Eichinger, L ;

Pachebat, JA ;

Glöckner, G ;

Rajandream, MA ;

Sucgang, R ;

Berriman, M ;

Song, J ;

Olsen, R ;

Szafranski, K ;

Xu, Q ;

Tunggal, B ;

Kummerfeld, S ;

Madera, M ;

Konfortov, BA ;

Rivero, F ;

Bankier, AT ;

Lehmann, R ;

Hamlin, N ;

Davies, R ;

Gaudet, P ;

Fey, P ;

Pilcher, K ;

Chen, G ;

Saunders, D ;

Sodergren, E ;

Davis, P ;

Kerhornou, A ;

Nie, X ;

Hall, N ;

Anjard, C ;

Hemphill, L ;

Bason, N ;

Farbrother, P ;

Desany, B ;

Just, E ;

Morio, T ;

Rost, R ;

Churcher, C ;

Cooper, J ;

Haydock, S ;

van Driessche, N ;

Cronin, A ;

Goodhead, I ;

Muzny, D ;

Mourier, T ;

Pain, A ;

Lu, M ;

Harper, D ;

Lindsay, R ;

Hauser, H .

NATURE, 2005, 435 (7038) :43-57

[7] Formins: signaling effectors for assembly and polarization of actin filaments [J].

Evangelista, M ;

Zigmond, S ;

Boone, C .

JOURNAL OF CELL SCIENCE, 2003, 116 (13) :2603-2611

[8] Formins direct Arp2/3-independent actin filament assembly to polarize cell growth in yeast [J].

Evangelista M. ;

Pruyne D. ;

Amberg D.C. ;

Boone C. ;

Bretscher A. .

Nature Cell Biology, 2002, 4 (1) :32-41

[9] Bni1p, a yeast formin linking Cdc42p and the actin cytoskeleton during polarized morphogenesis [J].

Evangelista, M ;

Blundell, K ;

Longtine, MS ;

Chow, CJ ;

Adames, N ;

Pringle, JR ;

Peter, M ;

Boone, C .

SCIENCE, 1997, 276 (5309) :118-122

[10] DGAP1, a homologue of rasGTPase activating proteins that controls growth, cytokinesis, and development in Dictyostelium discoideum [J].

Faix, J ;

Dittrich, W .

FEBS LETTERS, 1996, 394 (03) :251-257

← 1 2 3 4 →