The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia

被引：194

作者：

Schirenbeck, A

Bretschneider, T

Arasada, R

Schleicher, M

Faix, J

机构：

[1] Univ Munich, A Butenandt Inst Zellbiol, D-80336 Munich, Germany

[2] Max Planck Inst Biochem, AG Zelldynamik, D-82152 Martinsried, Germany

来源：

NATURE CELL BIOLOGY | 2005年 / 7卷 / 06期

关键词：

D O I：

10.1038/ncb1266

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Formins have important roles in the nucleation of actin and the formation of linear actin filaments, but their role in filopodium formation has remained elusive. Dictyostelium discoideum Diaphanous-related formin dDia2 is enriched at the tips of filopodia and interacts with profilin II and Rac1. An FH1FH2 fragment of dDia2 nucleated actin polymerization and removed capping protein from capped filament ends. Genetic studies showed that dDia2 is important for cell migration as well as the formation, elongation and maintenance of filopodia. Here we provide evidence that dDia2 specifically controls filopodial dynamics by regulating actin turnover at the barbed ends of actin filaments.

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页码：619 / U24

页数：8

相关论文

共 35 条

[11]

Faix J, 1998, J CELL SCI, V111, P3059

[12] Recruitment of cortexillin into the cleavage furrow is controlled by Rac1 and IQGAP-related proteins [J].

Faix, J ;

Weber, I ;

Mintert, U ;

Köhler, J ;

Lottspeich, F ;

Marriott, G .

EMBO JOURNAL, 2001, 20 (14) :3705-3715

[13] Requirement of a vasodilator-stimulated phosphoprotein family member for cell adhesion, the formation of filopodia, and chemotaxis in dictyostelium [J].

Han, YH ;

Chung, CY ;

Wessels, D ;

Stephens, S ;

Titus, MA ;

Soll, DR ;

Firtel, RA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (51) :49877-49887

[14]

HARTMANN H, 1989, J BIOL CHEM, V264, P12639

[15] DICTYOSTELIUM AMEBAS THAT LACK G-ACTIN-SEQUESTERING PROFILINS SHOW DEFECTS IN F-ACTIN CONTENT, CYTOKINESIS, AND DEVELOPMENT [J].

HAUGWITZ, M ;

NOEGEL, AA ;

KARAKESISOGLOU, J ;

SCHLEICHER, M .

CELL, 1994, 79 (02) :303-314

[16] CAPPING PROTEIN-LEVELS INFLUENCE ACTIN ASSEMBLY AND CELL MOTILITY IN DICTYOSTELIUM [J].

HUG, C ;

JAY, PY ;

REDDY, I ;

MCNALLY, JG ;

BRIDGMAN, PC ;

ELSON, EL ;

COOPER, JA .

CELL, 1995, 81 (04) :591-600

[17] ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum [J].

Kitayama, C ;

Uyedat, TQR .

JOURNAL OF CELL SCIENCE, 2003, 116 (04) :711-723

[18] The fission yeast cytokinesis formin Cdc12p is a barbed end actin filament capping protein gated by profilin [J].

Kovar, DR ;

Kuhn, JR ;

Tichy, AL ;

Pollard, TD .

JOURNAL OF CELL BIOLOGY, 2003, 161 (05) :875-887

[19] Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces [J].

Kovar, DR ;

Pollard, TD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (41) :14725-14730

[20] Critical role of Ena/VASP proteins for filopodia formation in neurons and in function downstream of netrin-1 [J].

Lebrand, C ;

Dent, EW ;

Strasser, GA ;

Lanier, LM ;

Krause, M ;

Svitkina, TM ;

Borisy, GG ;

Gertler, FB .

NEURON, 2004, 42 (01) :37-49

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