MALDI-TOF MS analysis of soluble PEG based multi-step synthetic reaction mixtures with automated detection of reaction failure

被引:17
作者
Enjalbal, C [1 ]
Ribière, P
Lamaty, F
Yadav-Bhatnagar, N
Martinez, J
Aubagnac, JL
机构
[1] Univ Montpellier 2, LAPP, UMR 5810, F-34095 Montpellier, France
[2] Univ Montpellier 1, LAPP, UMR 5810, Montpellier, France
[3] Aventis Pharma, F-93325 Romainville, France
关键词
D O I
10.1016/j.jasms.2005.01.020
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Macromolecules of tunable solubility, used to mimic inert insoluble materials while maintaining solution conditions, allowed the performance of efficient supported organic chemistry and facilitated in situ reaction monitoring. To satisfy the high throughput requirements of automated synthetic processes, organic syntheses carried out on bifunctional polyethylene glycol polymers (PEG(3400)-OH) were monitored step-by-step by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). A protocol was designed to control the ionization mechanism of such polymers exhibiting high affinity for alkali metal cations. Automated, rapid, and reliable data interpretation was performed by an in-house developed visual basic application relying on the sodiated ion accurate monoisotopic mass measurement. The methodology was illustrated through the monitoring of a six-step synthetic scheme. © 2005 American Society for Mass Spectrometry.
引用
收藏
页码:670 / 678
页数:9
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