The ERRα orphan nuclear receptor controls morphogenetic movements during zebrafish gastrulation

被引:20
作者
Bardet, PL [1 ]
Horard, B [1 ]
Laudet, V [1 ]
Vanacker, JM [1 ]
机构
[1] Ecole Normale Super Lyon, Lab Biol Mol Cellule, CNRS, UMR 5161,IFR 128 BioSci Lyon Gerland, F-69364 Lyon, France
关键词
gastrulation; epiboly; convergent-extension; ERR; orphan nuclear receptor;
D O I
10.1016/j.ydbio.2005.02.018
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Gastrulation is a process involving cellular commitment and movements whereby the three fundamental germ layers are established in vertebrates embryos. Estrogen Receptor-Related (ERR) alpha is a nuclear receptor displaying high sequence identity to the Estrogen Receptors (ERs). However, ERR alpha is unable to bind and to be regulated by estrogens or any natural ligand to date. Whereas recent studies have suggested roles for ERR alpha in bone and adipose tissue metabolism in the mouse, little is known about its roles during embryonic development. In zebrafish embryos, ERR alpha is expressed from the beginning of gastrulation at the margin of the blastoderm that represents the presumptive mesendoderm. Using loss of function (morpholinos or a dominant-negative version of the protein) and gain of function (mRNA injection) strategies, we show here that ERR alpha is involved in epilboly and convergent-extension (CE) processes in the zebrafish. Altogether, these results propose ERR alpha as a new regulator of morphogenetic movement during gastrulation, independently of cell fate determination. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码:102 / 111
页数:10
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