The mediator coactivator complex: functional and physical roles in transcriptional regulation

被引:109
作者
Lewis, BA
Reinberg, D [1 ]
机构
[1] Howard Hughes Med Inst, Piscataway, NJ 08854 USA
[2] Robert Wood Johnson Med Sch, Div Nucl Acids Enzymol, Dept Biochem, Piscataway, NJ 08854 USA
关键词
transcription regulation; mediator; coactivator;
D O I
10.1242/jcs.00734
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In vivo, the DNA is packed into chromatin and transcription is dependent upon activators that recruit other factors to reverse the repressive effects of chromatin. The response to activators requires additional factors referred to as coactivators. One such coactivator, mediator, is a multi-subunit complex capable of responding to different activators. It plays an key role in activation, bridging DNA-bound activators, the general transcriptional machinery, especially RNA polymerase II, and the core promoter. Its subunits are necessary for a variety of positive and negative regulatory processes and serve as the direct targets of activators themselves. In vivo and in vitro studies support various roles for mediator in transcription initiation, while structural studies demonstrate that it engages in multiple interactions with RNA polymerise II, and adopts conformations that are activator specific.
引用
收藏
页码:3667 / 3675
页数:9
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