Quantum Dot Technology in Flow Cytometry

被引：19

作者：

Chattopadhyay, Pratip K. ^{[1
]}

机构：

[1] NIAID, ImmunoTechnol Sect, Vaccine Res Ctr, NIH, Bethesda, MD 20892 USA

来源：

RECENT ADVANCES IN CYTOMETRY, PART A: INSTRUMENTATION, METHODS, FIFTH EDITION | 2011年 / 102卷

关键词：

LIVE CELLS; NANOCRYSTALS; FLUORESCENCE; FUTURE; SHELL; ASSAY;

D O I：

10.1016/B978-0-12-374912-3.00018-3

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The development of quantum dot (QD) technology represents one of the most dramatic advances in flow cytometry history, offering the opportunity for highly multiplexed experiments and allowing better resolution of dimly staining markers. Here, we guide users through the technical aspects of using QDs (including instrumentation and antibody conjugation), demonstrate why QDs are useful in multicolor flow cytometry, and describe some of the challenges investigators may face when adopting this technology.

引用

页码：463 / 477

页数：15

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