The use of quantum dot nanocrystals in multicolor flow cytometry

被引:28
作者
Chattopadhyay, Pratip K. [1 ]
Perfetto, Stephen P. [1 ]
Yu, Joanne [1 ]
Roederer, Mario [1 ]
机构
[1] NIH, ImmunoTechnol Sect, Vaccine Res Ctr, Bethesda, MD 20892 USA
关键词
RESONANCE ENERGY-TRANSFER; T-CELLS; FLUORESCENCE; BINDING; NANOPARTICLES; CYTOTOXICITY; POTENTIALS; ANTIGEN; DONORS;
D O I
10.1002/wnan.75
中图分类号
TB3 [工程材料学];
学科分类号
082905 [生物质能源与材料];
摘要
Because of their unique fluorescence properties, quantum dots (QDs) represent a promising new technology in the realm of multicolor flow cytometry. Although commercial reagents and applications for the technology are still in the early phases of their development, the strategies and considerations necessary for successful use are becoming known. This article discusses the value of QDs in multicolor flow cytometry, introduces strategies to successfully incorporate QDs into routine use, and highlights emerging applications of the technology. (C) 2010 John Wiley & Sons, Inc. WIREs Nanomed Nanobiotechnol 2010 2 334-348
引用
收藏
页码:334 / 348
页数:15
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