Neutralization of enteric coronaviruses with Escherichia coli cells expressing single-chain Fv-autotransporter fusions

被引：17

作者：

Veiga, E ^{[1
]}

de Lorenzo, V ^{[1
]}

Fernández, LA ^{[1
]}

机构：

[1] CSIC, Dept Microbial Biotechnol, Ctr Nacl Biotecnol, Madrid 28049, Spain

来源：

JOURNAL OF VIROLOGY | 2003年 / 77卷 / 24期

关键词：

D O I：

10.1128/JVI.77.24.13396-13398.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report here that fusions of single-chain antibodies (scFvs) to the autotransporter beta domain of the IgA protease of Neisseria gonorrhoeae are instrumental in locating virus-neutralizing activity on the cell surface of Escherichia Coli. E. coli cells displaying scFvs against the transmissible gastroenteritis coronavirus on their surface blocked in vivo the access of the infectious agent to cultured epithelial cells. This result raises prospects for antiviral strategies aimed at hindering the entry into target cells by bacteria that naturally colonize the same intestinal niches.

引用

页码：13396 / 13398

页数：3

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