Use of double-focusing sector field ICP-mass spectrometry in tracer experiments, aiming at the quantification of Mg2+ transport across the intestine of tilapia fish

被引:7
作者
De Wannemacker, G
Ronderos, A
Moens, L
Vanhaecke, F
Bijvelds, MJC
Kolar, ZI
机构
[1] Univ Ghent, Analyt Chem Lab, B-9000 Ghent, Belgium
[2] Delft Univ Technol, Interfac Reactor Inst, NL-2629 JB Delft, Netherlands
关键词
D O I
10.1039/b101183i
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Double-focusing sector field inductively coupled plasma mass spectrometry (ICP-MS) was used for the determination of Mg-26/Mg-24 isotope ratios in order to quantify intestinal Mg2+ transport in the fish tilapia (Oreochromis mossambicus). As the sample medium contained substantial amounts of C, Na, P, S, Cl and K, the occurrence of molecular (e.g., C-2(+), C2H+, CN+ and NaH+) and doubly charged (Ca-48(2+)) ions acid tailing of the Na-23(+) ion signal resulted in spectral overlap at low mass resolution. Application of a higher mass resolution (R = 3000), however, permitted accurate determination of ME isotope ratios. The usefulness of cool plasma conditions was also evaluated, but this approach was insufficient to resolve all interferences completely. After careful optimisation of the measurement parameters, it was possible to measure the Mg-26/Mg-24 isotope ratio with a relative standard deviation (RSD) of 0.2-0.5% (n = 10 x 30 s) at R = 3000. From the isotope ratio results obtained, net intestinal Mg2+ fluxes were calculated, providing evidence for secretory Mg2+ transport. Conclusively, the measurement of stable isotope ratios with sector field ICP-MS presents a sophisticated tool for the study of Mg2+ transport processes in biological systems that may provide an effective alternative for the use of radiotracers.
引用
收藏
页码:581 / 586
页数:6
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