Nucleoside diphosphate kinase III is localized to the inter-membrane space in plant mitochondria

被引:63
作者
Sweetlove, LJ
Mowday, B
Hebestreit, HF
Leaver, CJ
Millar, AH [1 ]
机构
[1] Univ Western Australia, Fac Med & Dent, Dept Biochem, Nedlands, WA 6009, Australia
[2] Univ Western Australia, Fac Agr, Plant Sci Grp, Nedlands, WA 6009, Australia
[3] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England
[4] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
基金
澳大利亚研究理事会; 英国生物技术与生命科学研究理事会;
关键词
plant mitochondrion; nucleoside diphosphate kinase; proteomic;
D O I
10.1016/S0014-5793(01)03069-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three types of nucleoside diphosphate kinases (NDPKs) are found in plants but the intra-cellular compartmentation of these proteins is not certain, especially the location of the recently identified type III proteins. Through the fractionation of plant mitochondria from potato and Arabidopsis, display of protein profiles by 2D gel electrophoresis, and identification by mass spectrometry, we present the first direct evidence that type III proteins are localized in the inter-membrane space of plant mitochondria. The possible metabolic functions of NDPK III are discussed in light of its sub-cellular localization. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:272 / 276
页数:5
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