A bacterial artificial chromosome library for sequencing the complete human genome

被引：205

作者：

Osoegawa, K ^{[1
]}

Mammoser, AG ^{[1
]}

Wu, CY ^{[1
]}

Frengen, E ^{[1
]}

Zeng, CJ ^{[1
]}

Catanese, JJ ^{[1
]}

de Jong, PJ ^{[1
]}

机构：

[1] Roswell Pk Canc Inst, Dept Canc Genet, Buffalo, NY 14263 USA

来源：

GENOME RESEARCH | 2001年 / 11卷 / 03期

关键词：

D O I：

10.1101/gr.169601

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large average insert size (178 kb) has been constructed to provide the intermediate substrate for the international genome sequencing effort. The DNA was obtained from a a;ingle anonymous volunteer, whose identity was protected through a double-blind donor selection protocol. DNA fragments were generated by partial digestion with EcoRI (library segments 1-4: 24-fold) and Mbol (segment 5: sixfold) and cloned into the pBACe3.6 and pTARBACl vectors, respectively. The quality of the library was assessed by extensive analysis of 169 clones for rearrangements and artifacts. Eighteen BACs (11%) revealed minor insert rearrangements, and none was chimeric. This BAC library, designated as "RPCI-11," has been used widely as the central resource for insert-end sequencing, clone fingerprinting, high-throughput sequence analysis and as a source of mapped clones for diagnostic and functional studies.

引用

页码：483 / 496

页数：14

共 54 条

[31]

Osoegawa K, 2000, GENOME RES, V10, P116

[32] An improved approach for construction of bacterial artificial chromosome libraries [J].

Osoegawa, K ;

Woon, PY ;

Zhao, BH ;

Frengen, E ;

Tateno, M ;

Catanese, JJ ;

de Jong, PJ .

GENOMICS, 1998, 52 (01) :1-8

[33]

OSOEGAWA K, 1999, CURRENT PROTOCOLS HU

[34] Database divisions and homology search files: A guide for the perplexed [J].

Ouellette, BFF ;

Boguski, MS .

GENOME RESEARCH, 1997, 7 (10) :952-955

[35] High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays [J].

Pinkel, D ;

Seagraves, R ;

Sudar, D ;

Clark, S ;

Poole, I ;

Kowbel, D ;

Collins, C ;

Kuo, WL ;

Chen, C ;

Zhai, Y ;

Dairkee, SH ;

Ljung, BM ;

Gray, JW ;

Albertson, DG .

NATURE GENETICS, 1998, 20 (02) :207-211

[36] Correction of deafness in shaker-2 mice by an unconventional myosin in a BAC transgene [J].

Probst, FJ ;

Fridell, RA ;

Raphael, Y ;

Saunders, TL ;

Wang, AH ;

Liang, Y ;

Morell, RJ ;

Touchman, JW ;

Lyons, RH ;

Noben-Trauth, K ;

Friedman, TB ;

Camper, SA .

SCIENCE, 1998, 280 (5368) :1444-1447

[37] A sequence-ready physical map of a region of 12q24.1 [J].

Renault, B ;

Hovnanian, A ;

Bryce, S ;

Chang, JJ ;

Lau, S ;

Sakuntabhai, A ;

Monk, S ;

Carter, S ;

Ross, CJD ;

Pang, J ;

Twells, R ;

Chamberlain, S ;

Monaco, AP ;

Strachan, T ;

Kucherlapati, R .

GENOMICS, 1997, 45 (02) :271-278

[38]

Sambrook J., 1989, MOL CLONING

[39]

Schmitt H, 1998, INT J MOL MED, V1, P569

[40]

Shapira Stuart K., 1998, Current Opinion in Pediatrics, V10, P622

← 1 2 3 4 5 6 →