Mitochondrial localization of Arabidopsis thaliana Isu Fe-S scaffold proteins

被引：37

作者：

Léon, S

Touraine, B

Briat, JF

Lobréaux, S

机构：

[1] Univ Montpellier 2, INRA, CNRS, UMR 5004, F-34060 Montpellier, France

[2] Ecole Natl Super Agron, F-34060 Montpellier, France

来源：

FEBS LETTERS | 2005年 / 579卷 / 09期

关键词：

plant; iron-sulfur cluster; mitochondria; plastid;

D O I：

10.1016/j.febslet.2005.02.038

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Isu are scaffold proteins involved in iron-sulfur cluster biogenesis and playing a key role in yeast mitochondria and Escherichia coli. In this work, we have characterized the Arabidopsis thaliana Isu gene family. AtIsul,2,3 genes encode polypeptides closely related to their bacterial and eukaryotic counterparts. AtIsu expression in a Saccharomyces cerevisiae Delta isu1 Delta nfu1 thermosensitive mutant led to the growth restoration of this strain at 37 degrees C. Using Isu-GFP fusions expressed in leaf protoplasts and immunodetection in organelle extracts, we have shown that Arabidopsis Isu proteins are located only into mitochondria, supporting the existence of an Isu-independent Fe-S assembly machinery in plant plastids. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：1930 / 1934

页数：5

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