Quantification of human immunoglobulin G immobilized on gold-coated silicon chip for biosensing applications

被引：10

作者：

Vashist, S. K. ^{[1
]}

Raiteri, R. ^{[1
]}

Tewari, R. ^{[2
]}

Bajpai, R. P. ^{[3
]}

Bharadwaj, L. M. ^{[3
]}

机构：

[1] Univ Genoa, DIBE, Neuroengn & Bionanotechnol Grp, Via Opera Pia 11A, I-16145 Genoa, Italy

[2] Panjab Univ, Dept Biotechnol, Chandigarh 160014, India

[3] Cent Sci Instruments Org, Biomol Elect & Nanotechnol Div, Chandigarh 160030, India

来源：

INTERNATIONAL MEMS CONFERENCE 2006 | 2006年 / 34卷

关键词：

D O I：

10.1088/1742-6596/34/1/133

中图分类号：

TP [自动化技术、计算机技术];

学科分类号：

0812 ;

摘要：

The most important aspect of biosensor development with high sensitivity is the oriented immobilization of antibodies on the solid substrate. Human IgG was immobilized on gold-coated silicon employing protein A, protein G and neutravidin immobilization procedures. The amount of human IgG immobilized was analyzed by 3, 3', 4, 4'-tetramethyl benzidine (TMB) substrate assay and was maximum when protein A immobilization procedure was followed. Human IgG coated biosensing surface was regenerated by treatment with glycine-HCl buffer (50 mM, pH 2.2). Atomic force microscopy was used to analyze the distribution of biomolecules immobilized on gold-coated silicon. Our study indicates that human IgG molecules were uniformly bound to gold-coated silicon by protein A immobilization procedure.

引用

页码：806 / 811

页数：6