Cellular electrophysiology of canine pulmonary vein cardiomyocytes: action potential and ionic current properties

被引:217
作者
Ehrlich, JR
Cha, TJ
Zhang, LM
Chartier, D
Melnyk, P
Hohnloser, SH
Nattel, S
机构
[1] Montreal Heart Inst, Res Ctr, Montreal, PQ H1T 1C8, Canada
[2] Montreal Heart Inst, Dept Med, Montreal, PQ H1T 1C8, Canada
[3] Univ Montreal, Dept Pharmacol & Therapeut, Montreal, PQ, Canada
[4] Univ Montreal, Dept Pathol, Montreal, PQ, Canada
[5] McGill Univ, Dept Pathol, Montreal, PQ, Canada
[6] Goethe Univ Frankfurt, D-6000 Frankfurt, Germany
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2003年 / 551卷 / 03期
关键词
D O I
10.1113/jphysiol.2003.046417
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Pulmonary vein (PV) cardiomyocytes play an important role in atrial fibrillation; however, little is known about their specific cellular electrophysiological properties. We applied standard microelectrode recording and whole-cell patch-clamp to evaluate action potentials and ionic currents in canine PVs and left atrium (LA) free wall. Resting membrane potential (RMP) averaged -66 +/- 1 mV in PVs and -74 +/- 1 mV in LA (P < 0.0001) and action potential amplitude averaged 76 2 mV in PVs vs. 95 2 mV in LA (P < 0.0001). PVs had smaller maximum phase 0 upstroke velocity (V-max : 98 +/- 9 vs. 259 +/- 16 V s(-1), P < 0.0001) and action potential duration (APD): e.g. at 2 Hz, APD to 90% repolarization in PVs was 84% of LA (P < 0.05). Na+ current density under voltage-clamp conditions was similar in PV and LA, suggesting that smaller V-max in PVs was due to reduced RMP. Inward rectifier current density in the PV cardiomyocytes was similar to58% that in the LA, potentially accounting for the less negative RMP in PVs. Slow and rapid delayed rectifier currents were greater in the PV (by similar to60 and similar to50%, respectively), whereas transient outward K+ current and L-type Ca2+ current were significantly smaller (by similar to25 and similar to30%, respectively). Na+-Ca2+-exchange (NCX) current and T-type Ca2+ current were not significantly different. In conclusion, PV cardiomyocytes have a discrete distribution of transmembrane ion currents associated with specific action potential properties, with potential implications for understanding PV electrical activity in cardiac arrhythmias.
引用
收藏
页码:801 / 813
页数:13
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