Flank matrix attachment regions (MARs) from chicken, bean, yeast or tobacco do not prevent homology-dependent trans-silencing in transgenic tobacco plants

被引:22
作者
Vaucheret, H [1 ]
Elmayan, T
Thierry, D
van der Geest, A
Hall, T
Conner, AJ
Mlynarova, L
Nap, JP
机构
[1] INRA, Biol Cellulaire Lab, F-78026 Versailles, France
[2] Texas A&M Univ, Inst Dev & Mol Biol, College Stn, TX 77843 USA
[3] DLO, CPRO, Dept Biol Mol, NL-6700 AA Wageningen, Netherlands
来源
MOLECULAR AND GENERAL GENETICS | 1998年 / 259卷 / 04期
关键词
matrix attachment regions; homology-dependents gene silencing;
D O I
10.1007/s004380050827
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of flanking matrix attachment regions (MARs) on homology-dependent trans-silencing was tested using two strong tuans-silencing loci. The transgenic tobacco line 271 carries at a single locus a p35S-RiN-tNos transgene which is able to silence, in trans and at the transcriptional level, the expression of any p35S-driven transgene irrespective of its position. The transgenic tobacco line 6b8 carries at a single locus a p35S-uidA-tRbcS transgene which is able to silence in tl ws, at the post-transcriptional level, the expression of any uidA-expressing transgene irrespective of its position. Various transgenic tobacco lines carrying a target p35S-uidA-tNos transgene, flanked on each side by MARs from chicken, bean, yeast or tobacco, were crossed with lines carrying the 271 and 6b8 loci. Expression of the target transgene was silenced in all hybrids, irrespective of the presence or absence of MAR sequences. These results therefore demonstrate that MARs are not able to protect transgene expression from strong silencing loci that act in trans.
引用
收藏
页码:388 / 392
页数:5
相关论文
共 30 条
[21]  
MLYNAROVA L, 1994, PLANT CELL, V6, P417, DOI 10.1105/tpc.6.3.417
[22]  
MLYNAROVA L, 1995, PLANT CELL, V7, P599, DOI 10.1105/tpc.7.5.599
[23]  
Nap JP, 1997, GENETICS, V147, P315
[24]   Gene silencing mediated by promoter homology occurs at the level of transcription and results in meiotically heritable alterations in methylation and gene activity [J].
Park, YD ;
Papp, I ;
Moscone, EA ;
Iglesias, VA ;
Vaucheret, H ;
Matzke, AJM ;
Matzke, MA .
PLANT JOURNAL, 1996, 9 (02) :183-194
[25]   REVERSIBLE INACTIVATION OF A TRANSGENE IN ARABIDOPSIS-THALIANA [J].
SCHEID, OM ;
PASZKOWSKI, J ;
POTRYKUS, I .
MOLECULAR AND GENERAL GENETICS, 1991, 228 (1-2) :104-112
[26]   A NUCLEAR-DNA ATTACHMENT ELEMENT MEDIATES ELEVATED AND POSITION-INDEPENDENT GENE ACTIVITY [J].
STIEF, A ;
WINTER, DM ;
STRATLING, WH ;
SIPPEL, AE .
NATURE, 1989, 341 (6240) :343-345
[27]  
VANDERGEEST AHM, 1994, PLANT J, V6, P413, DOI 10.1046/j.1365-313X.1994.06030413.x
[28]  
VAUCHERET H, 1993, CR ACAD SCI III-VIE, V316, P1471
[29]   RNA-DIRECTED DE-NOVO METHYLATION OF GENOMIC SEQUENCES IN PLANTS [J].
WASSENEGGER, M ;
HEIMES, S ;
RIEDEL, L ;
SANGER, HL .
CELL, 1994, 76 (03) :567-576
[30]   RIGS (repeat-induced gene silencing) in Arabidopsis is transcriptional and alters chromatin configuration [J].
Ye, F ;
Signer, ER .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (20) :10881-10886