Genetic characterization of Entamoeba dispar isolates in Northeast Brazil

被引：10

作者：

Pinheiro, SMB

Maciel, RF

Morais, MA

Aca, IS

Carvalho, LB

Coimbra, MRM

机构：

[1] Univ Fed Rural Pernambuco, Dept Pesca, LAGA, BR-52171900 Recife, PE, Brazil

[2] Univ Fed Pernambuco, Lab Imunopatol Keizo Asami, BR-50670901 Recife, PE, Brazil

[3] Univ Fed Pernambuco, Dept Trop Med, BR-50670901 Recife, PE, Brazil

[4] Univ Fed Pernambuco, Dept Genet, BR-50670901 Recife, PE, Brazil

[5] Univ Fed Pernambuco, Dept Bioquim, BR-50670901 Recife, PE, Brazil

来源：

ACTA TROPICA | 2005年 / 94卷 / 01期

关键词：

Entamoeba dispar; molecular characterization; northeast Brazil;

D O I：

10.1016/j.actatropica.2005.01.012

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The genetic variability of Entamoeba dispar strains was investigated in 39 positive isolates on a survey of 1783 individuals from two different cities of Northeast Brazil (Recife and Macaparana) using two polymorphic species-specific loci (loci 1-2 and 5-6). A combinatory clustering analysis revealed no geographical correlation and remarkable genetic polymorphism among all the isolates examined. Nevertheless, a comparison of the frequency of eight individual PCR products, shared by both Recife and Macaparana populations, for the two loci, showed that only one product of locus 5-6 was significantly different between the two cities. These results suggested that the Macaparana population is infected by similar strains and that locus 5-6 shows potential in assaying questions related to the molecular epidemiology of this region. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：35 / 40

页数：6