Reduced mitochondrial DNA transcription in senescent rat heart

被引：32

作者：

Andreu, AL

Arbos, MA

Perez-Martos, A

Lopez-Perez, MJ

Asin, J

Lopez, N

Montoya, J

Schwartz, S

机构：

[1] Hosp Gen Valle Hebron, Ctr Invest Bioquim & Biol Mol, E-08035 Barcelona, Spain

[2] Univ Zaragoza, Dept Bioquim & Biol Mol & Celular, E-50013 Zaragoza, Spain

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 252卷 / 03期

关键词：

mitochondrial DNA; aging; transcription in vitro; OXPHOS system; oxidative damage; gene expression;

D O I：

10.1006/bbrc.1998.9703

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We studied the effect of senescence on mitochondrial DNA (mtDNA) transcription with an in organello system using intact isolated rat heart mitochondria. A comparison of the electrophoretic patterns of mtDNA transcription products in young, adult and senescent rats showed an age-related reduction in newly-synthesized mitochondrial RNAs that reflects a decrease in the synthesis rate. These results correlate with the enzyme activities of the oxidative phosphorylation complexes I and IV, that are partially encoded by the mitochondrial genome. In addition, an age-related increase in the protein carbonyl content of the mitochondrial membranes was observed in senescent mitochondria suggesting an accumulation of mitochondrial oxidative damage. This reduction in the mtDNA transcriptional rate in the heart of senescent animals suggests that this could be one of the molecular bases underlying senescence of the myocardium. (C) 1998 Academic Press.

引用

页码：577 / 581

页数：5

共 37 条

[21] CARDIOVASCULAR REGULATORY MECHANISMS IN ADVANCED AGE
LAKATTA, EG
[J]. PHYSIOLOGICAL REVIEWS, 1993, 73 (02) : 413 - 467
[22] LEVINE RL, 1990, METHOD ENZYMOL, V186, P464
[23] AGING - CAUSES AND DEFENSES
MARTIN, GR
DANNER, DB
HOLBROOK, NJ
[J]. ANNUAL REVIEW OF MEDICINE, 1993, 44 : 419 - 429
[24] AN UPDATE ON THE MITOCHONDRIAL-DNA MUTATION HYPOTHESIS OF CELL AGING
MIQUEL, J
[J]. MUTATION RESEARCH, 1992, 275 (3-6): : 209 - 216
[25] THE PATTERN OF TRANSCRIPTION OF THE HUMAN MITOCHONDRIAL RIBOSOMAL-RNA GENES REVEALS 2 OVERLAPPING TRANSCRIPTION UNITS
MONTOYA, J
GAINES, GL
ATTARDI, G
[J]. CELL, 1983, 34 (01) : 151 - 159
[26] Genetic and functional changes in mitochondria associated with aging
Ozawa, T
[J]. PHYSIOLOGICAL REVIEWS, 1997, 77 (02) : 425 - 464
[27] Ragan CI, 1988, MITOCHONDRIA PRACTIC, P79
[28] ACCUMULATION OF DELETIONS IN HUMAN MITOCHONDRIAL-DNA DURING NORMAL AGING - ANALYSIS BY QUANTITATIVE PCR
SIMONETTI, S
CHEN, X
DIMAURO, S
SCHON, EA
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1992, 1180 (02) : 113 - 122
[29] Oxidative stress, caloric restriction, and aging
Sohal, RS
Weindruch, R
[J]. SCIENCE, 1996, 273 (5271) : 59 - 63
[30] MITOCHONDRIAL PRODUCTION OF PRO-OXIDANTS AND CELLULAR SENESCENCE
SOHAL, RS
BRUNK, UT
[J]. MUTATION RESEARCH, 1992, 275 (3-6): : 295 - 304

← 1 2 3 4 →