DNA hybridization measurement by self-sensing piezoresistive microcantilevers in CMOS biosensor

被引：24

作者：

Yang, S. M. ^{[1
]}

Chang, C. ^{[1
]}

Yin, T. I. ^{[1
]}

Kuo, P. L. ^{[2
]}

机构：

[1] Natl Cheng Kung Univ, Dept Aeronaut & Astronaut, Taipei 701, Taiwan

[2] Natl Cheng Kung Univ, Coll Med, Dept Obstet & Gynecol, Taipei 701, Taiwan

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2008年 / 130卷 / 02期

关键词：

smicrocantilever; CMOS biosensor; DNA chip;

D O I：

10.1016/j.snb.2007.10.072

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Understanding the mechanism of how biological reactions produce mechanical loadings is fundamental to biomedical developments. A CMOS biosensor chip is developed to measure in situ the induced surface stress change by DNA hybridization. For 20-mer thiol-modified single stranded DNA (ssDNA), the mechanism of ssDNA attached to gold surface via a sulfur-gold linkage can be investigated by using the Langmuir adsorption model. Experimental results indicate that the immobilization response is less than I s, the total number of ssDNA molecules on the cantilever is about 3 x 10(11), and the induced surface stress is 0. 15 N/m. The surface stress sensitivity of the sensor is about 3.5 x 10(-5) m/N. The estimated adsorption rate of the ssDNA is 0.005 s(-1). The biosensor is capable of discriminating complimentary molecular targets and thus may provide a powerful platform for high throughput real-time analysis of DNA. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：674 / 681

页数：8

共 24 条

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