Two different strategies for the fluorimetric determination of piroxicam in serum

被引:118
作者
Arancibia, JA [1 ]
Escandar, GM [1 ]
机构
[1] Univ Nacl Rosario, Dept Quim Analit, Fac Ciencias Bioquim & Farmaceut, RA-2000 Rosario, Argentina
关键词
spectrofluorimetry; three-way multivariate calibration; solid-phase-extraction; piroxicam;
D O I
10.1016/S0039-9140(03)00221-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Two different spectrofluorimetric methods for the determination of piroxicam (PX) in serum are presented and discussed. One of them is based on the use of three-way fluorescence data and multivariate calibration performed with parallel factor analysis (PARAFAC) and self-weighted alternating trilinear decomposition (SWATLD). This methodology exploits the so-called second-order advantage of the three-way data, allowing to obtain the concentration of the studied analyte in the presence of any number of uncalibrated (serum) components. The method was developed following two different procedures: internal standard addition and external calibration with standard solutions, which were compared and discussed. The second approach investigated is based on the combination of solid-phase extraction (SPE) and room temperature fluorimetry. Both methods here presented yield satisfactory results. The concentration range in which PX could be determined in serum was 1-10 mug ml(-1). The limits of quantification for the experimental solutions using the chemometric approach were 0.09 mug ml(-1) for the standard addition mode and 0.12 mug ml(-1) using external calibration (both for PARAFAC and SWATLD algorithms). In the solid-surface fluorimetric method, the calibration graph was linear up to 0.22 mug ml(-1) and the limit of quantification was 0.02 mug ml(-1.) (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:1113 / 1121
页数:9
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