Tunable Hydrophobicity in DNA Micelles: Design, Synthesis, and Characterization of a New Family of DNA Amphiphiles

被引：50

作者：

Anaya, Milena ^{[1
]}

Kwak, Minseok ^{[2
]}

Musser, Andrew J. ^{[2
]}

Muellen, Klaus ^{[1
]}

Herrmann, Andreas ^{[2
]}

机构：

[1] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

[2] Univ Groningen, Dept Polymer Chem, Zernike Inst Adv Mat, NL-9747 AG Groningen, Netherlands

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2010年 / 16卷 / 43期

关键词：

DNA hybridization; nanoparticles; nucleic acids; nucleolipids; self-assembly; BLOCK-COPOLYMER MICELLES; SYBR-GREEN-I; ATOMIC-FORCE MICROSCOPY; SINGLE-STRANDED-DNA; MOLECULAR RECOGNITION; TRIPLEX FORMATION; PHYSIOLOGICAL PH; OLIGONUCLEOTIDES; FLUORESCENCE; STABILITY;

D O I：

10.1002/chem.201001816

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This work describes the synthesis and characterization of a new family of DNA amphiphiles containing modified nucleobases. The hydrophobicity was imparted by the introduction of a dodec-1-yne chain at the 5-position of the uracil base, which allowed precise and simple tuning of the hydrophobic properties through solid-phase DNA synthesis. The micelles formed from these modified DNA sequences were characterized by atomic force microscopy, dynamic light scattering, and polyacrylamide gel electrophoresis. These experiments revealed the role of the quantity and location of the hydrophobic units in determining the morphology and stability of the micelles. The effects of hybridization on the physical characteristics of the DNA micelles were also studied; these results showed potential for the sequence-specific noncovalent functionalization of the self-assembled aggregates.

引用

页码：12852 / 12859

页数：8

共 59 条

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