The interaction of boronic acid-substituted viologens with pyranine: The effects of quencher charge on fluorescence quenching and glucose response

被引：70

作者：

Cordes, DB ^{[1
]}

Gamsey, S ^{[1
]}

Sharrett, Z ^{[1
]}

Miller, A ^{[1
]}

Thoniyot, P ^{[1
]}

Wessling, RA ^{[1
]}

Singaram, B ^{[1
]}

机构：

[1] Univ Calif Santa Cruz, Dept Chem & Biochem, Santa Cruz, CA 95064 USA

来源：

LANGMUIR | 2005年 / 21卷 / 14期

关键词：

D O I：

10.1021/la050219x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The fluorescence sensing of several monosaccharides using boronic acid-substituted viologen quenchers in combination with the fluorescent dye pyranine (HPTS) is reported. In this two-component sensing system, fluorescence quenching by the viologen is modulated by monosaccharides to provide a fluorescence signal. A series of viologen quenchers with different charges were prepared and tested for their ability both to quench the fluorescence of HPTS and to sense changes in glucose concentration in aqueous solution at pH 7.4. Both quenching efficiency and sugar sensing were found to be strongly dependent upon viologen charge. The molar ratio between HPTS and each of the viologen quenchers was varied in order to obtain an optimal ratio that provided a fairly linear fluorescence signal across a physiological glucose concentration range. Both the quenching and sugar sensing results are explained by electrostatic interaction between dye and quencher.

引用

页码：6540 / 6547

页数：8

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