Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica

被引:180
作者
Xue, CH [1 ]
Fang, Y [1 ]
Lin, H [1 ]
Chen, L [1 ]
Li, ZJ [1 ]
Deng, D [1 ]
Lu, CX [1 ]
机构
[1] Ocean Univ Qingdao, Dept Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China
关键词
antioxidant; chemical characters; fucoidan; Laminaria japonica; LDL; low-density lipoprotein;
D O I
10.1023/A:1008103611522
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A low molecular weight sulfated polysaccharide (L-A) was prepared by mild acid hydrolysis of crude fucoidan (F-A) from Laminaria japonica. In comparison with F-A, L-A had a lower proportion of fucose residues, but a similar proportion of sulfate. The galactose content of both fractions was relatively high, especially for L-A (57%). The antioxidant properties of the two polysaccharides were studied using two low-density lipoprotein oxidation systems. L-A had a stronger effect against low-density lipoprotein oxidation in both systems. F-A inhibited the AAPH-induced low-density lipoprotein oxidation, but had little effect on the Cu++-induced system due to its large molecular mass. The active sulfated fraction L-A, containing galactose, mannose and fucose (about 9: 2: 2) is reported here for the first time.
引用
收藏
页码:67 / 70
页数:4
相关论文
共 33 条
[21]  
PUHL H, 1994, METHOD ENZYMOL, V233, P425
[22]  
REISSIG JL, 1955, J BIOL CHEM, V217, P959
[23]   FREE RADICAL-MEDIATED CHAIN OXIDATION OF LOW-DENSITY-LIPOPROTEIN AND ITS SYNERGISTIC INHIBITION BY VITAMIN-E AND VITAMIN-C [J].
SATO, K ;
NIKI, E ;
SHIMASAKI, H .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 279 (02) :402-405
[24]   INHIBITORY EFFECT OF OVERSULFATED FUCOIDAN ON INVASION THROUGH RECONSTITUTED BASEMENT-MEMBRANE BY MURINE LEWIS LUNG-CARCINOMA [J].
SOEDA, S ;
ISHIDA, S ;
SHIMENO, H ;
NAGAMATSU, A .
JAPANESE JOURNAL OF CANCER RESEARCH, 1994, 85 (11) :1144-1150
[25]   DECREASE IN REACTIVE AMINO-GROUPS DURING OXIDATION OR ENDOTHELIAL-CELL MODIFICATION OF LDL - CORRELATION WITH CHANGES IN RECEPTOR-MEDIATED CATABOLISM [J].
STEINBRECHER, UP ;
WITZTUM, JL ;
PARTHASARATHY, S ;
STEINBERG, D .
ARTERIOSCLEROSIS, 1987, 7 (02) :135-143
[26]  
TAICHI U, 1980, AGR BIOL CHEM TOKYO, V44, P1965
[27]  
Tutour BL, 1998, J APPL PHYCOL, V10, P121
[28]   Influence of chondroitin sulfate charge density, sulfate group position, and molecular mass on Cu2+-mediated oxidation of human low-density lipoproteins:: Effect of normal human plasma-derived chondroitin sulfate [J].
Volpi, N ;
Tarugi, P .
JOURNAL OF BIOCHEMISTRY, 1999, 125 (02) :297-304
[29]   Antioxidative activities of several marine polysaccharides evaluated in a phosphatidylcholine-liposomal suspension and organic solvents [J].
Xue, CH ;
Yu, GL ;
Hirata, T ;
Terao, J ;
Lin, H .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (02) :206-209
[30]  
YAMAMOTO I, 1981, JPN J EXP MED, V51, P187