Fully automated four-column capillary LC-MS system for maximizing throughput in proteomic analyses

被引：125

作者：

Livesay, Eric A. ^{[1
]}

Tang, Keqi ^{[1
]}

Taylor, Beverley K. ^{[1
]}

Buschbach, Michael A. ^{[1
]}

Hopkins, Derek F. ^{[1
]}

LaMarche, Brian L. ^{[1
]}

Zhao, Rui ^{[1
]}

Shen, Yufeng ^{[1
]}

Orton, Daniel J. ^{[1
]}

Moore, Ronald J. ^{[1
]}

Kelly, Ryan T. ^{[1
]}

Udseth, Harold R. ^{[1
]}

Smith, Richard D. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Div Biol Sci, Environm Mol Sci Lab, Richland, WA 99354 USA

来源：

ANALYTICAL CHEMISTRY | 2008年 / 80卷 / 01期

关键词：

D O I：

10.1021/ac701727r

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe a four-column, high-pressure capillary liquid chromatography (ILC) system for robust, high-throughput liquid chromatography-mass specbrometry (LC-MS(/MS)) analyses. This system performs multiple LC separations in parallel, but staggers each of them such that the data-rich region of each separation is sampled sequentially. By allowing nearly continuous data acquisition, this design maximizes the use of the mass spectrometer. Each analytical column is connected to a corresponding ESI emitter in order to avoid the use of postcolumn switching and associated dead volume issues. Encoding translation stages are employed to sequentially position the emitters at the MS inlet. The high reproducibility of this system is demonstrated using consecutive analyses of global tryptic digest of the microbe Shewanella oneidensis.

引用

页码：294 / 302

页数：9

共 30 条

[1]

Bayliss MK, 2000, RAPID COMMUN MASS SP, V14, P2039, DOI 10.1002/1097-0231(20001115)14:21<2039::AID-RCM130>3.0.CO

[2]

2-2

[3] An automated high performance capillary liquid chromatography-Fourier transform ion cyclotron resonance mass spectrometer for high-throughput proteomics [J].

Belov, ME ;

Anderson, GA ;

Wingerd, MA ;

Udseth, HR ;

Tang, KQ ;

Prior, DC ;

Swanson, KR ;

Buschbach, MA ;

Strittmatter, EF ;

Moore, RJ ;

Smith, RD .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2004, 15 (02) :212-232

[4] Rapid bioanalysis of vancomycin in serum and urine by high-performance liquid chromatography tandem mass spectrometry using on-line sample extraction and parallel analytical columns [J].

Cass, RT ;

Villa, JS ;

Karr, DE ;

Schmidt, DE .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (06) :406-412

[5]

de Biasi V, 1999, RAPID COMMUN MASS SP, V13, P1165, DOI 10.1002/(SICI)1097-0231(19990630)13:12<1165::AID-RCM638>3.0.CO

[6]

2-4

[7] High-speed gradient parallel liquid chromatography/tandem mass spectrometry with fully automated sample preparation for bioanalysis: 30 seconds per sample from plasma [J].

Deng, Y ;

Wu, JT ;

Lloyd, TL ;

Chi, CL ;

Olah, TV ;

Unger, SE .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2002, 16 (11) :1116-1123

[8] Multiple-sprayer tandem mass spectrometry with parallel high flow extraction and parallel separation for high-throughput quantitation in biological fluids [J].

Deng, YZ ;

Zeng, H ;

Unger, SE ;

Wu, JT .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (17) :1634-1640

[9] Higher-throughput screening for Caco-2 permeability utilizing a multiple sprayer liquid chromatography/tandem mass spectrometry system [J].

Fung, EN ;

Chu, IH ;

Li, C ;

Liu, TT ;

Soares, A ;

Morrison, R ;

Nomeir, AA .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2003, 17 (18) :2147-2152

[10] Current two-dimensional electrophoresis technology for proteomics [J].

Görg, A ;

Weiss, W ;

Dunn, MJ .

PROTEOMICS, 2004, 4 (12) :3665-3685

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