A multiprotein complex that interacts with RNA polymerase II elongator

被引：59

作者：

Li, Y

Takagi, Y

Jiang, YW

Tokunaga, M

Erdjument-Bromage, H

Tempst, P

Kornberg, RD

机构：

[1] Stanford Univ, Dept Biol Struct, Sch Med, Stanford, CA 94305 USA

[2] Texas A&M Univ, Dept Med Biochem & Genet, Hlth Sci Ctr, College Stn, TX 77843 USA

[3] Kagoshima Univ, Fac Agr, Kagoshima 8900065, Japan

[4] Mem Sloan Kettering Canc Ctr, Program Mol Biol, New York, NY 10021 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2001年 / 276卷 / 32期

关键词：

D O I：

10.1074/jbc.C100274200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A three-subunit Hap complex that interacts with the RNA polymerase If Elongator was isolated fi-om yeast. Deletions of genes for two Hap subunits, HAP1 and HAP3, confer pGKL killer-insensitive and weak Elongator phenotypes. Preferential interaction of the Hap complex with free rather than RNA polymerase II-associated Elongator suggests a role in the regulation of Elongator activity.

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收藏

页码：29628 / 29631

页数：4

相关论文

共 21 条

[1] Histone deacetylases: transcriptional repression with SINers and NuRDs [J].

Ayer, DE .

TRENDS IN CELL BIOLOGY, 1999, 9 (05) :193-198

[2] The many HATs of transcription coactivators [J].

Brown, CE ;

Lechner, T ;

Howe, L ;

Workman, JL .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (01) :15-19

[3] Essential role of Swp73p in the function of yeast Swi/Snf complex [J].

Cairns, BR ;

Levinson, RS ;

Yamamoto, KR ;

Kornberg, RD .

GENES & DEVELOPMENT, 1996, 10 (17) :2131-2144

[4] A human RNA polymerase II complex containing factors that modify chromatin structure [J].

Cho, H ;

Orphanides, G ;

Sun, XQ ;

Yang, XJ ;

Ogryzko, V ;

Lees, E ;

Nakatani, Y ;

Reinberg, D .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (09) :5355-5363

[5] Phosphorylation of linker histone H1 regulates gene expression in vivo by creating a charge patch [J].

Dou, YL ;

Gorovsky, MA .

MOLECULAR CELL, 2000, 6 (02) :225-231

[6] Examination of micro-tip reversed-phase liquid chromatographic extraction of peptide pools for mass spectrometric analysis [J].

Erdjument-Bromage, H ;

Lui, M ;

Lacomis, L ;

Grewal, A ;

Annan, RS ;

McNulty, DE ;

Carr, SA ;

Tempst, P .

JOURNAL OF CHROMATOGRAPHY A, 1998, 826 (02) :167-181

[7] The Elp2 subunit of elongator and elongating RNA polymerase II holoenzyme is a WD40 repeat protein [J].

Fellows, J ;

Erdjument-Bromage, H ;

Tempst, P ;

Svejstrup, JQ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (17) :12896-12899

[8] Isolation and genetic characterization of pGKL killer-insensitive mutants (iki) from Saccharomyces cerevisiae [J].

Kishida, M ;

Tokunaga, M ;

Katayose, Y ;

Yajima, H ;

KawamuraWatabe, A ;

Hishinuma, F .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (05) :798-801

[9]

Li Y, 1996, METHOD ENZYMOL, V273, P172

[10] YEAST GLOBAL TRANSCRIPTIONAL REGULATORS SIN4 AND RGR1 ARE COMPONENTS OF MEDIATOR COMPLEX RNA-POLYMERASE-II HOLOENZYME [J].

LI, Y ;

BJORKLUND, S ;

JIANG, YW ;

KIM, YJ ;

LANE, WS ;

STILLMAN, DJ ;

KORNBERG, RD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (24) :10864-10868