De novo high-resolution protein structure determination from sparse spin-labeling EPR data

被引:108
作者
Alexander, Nathan [1 ,3 ]
Bortolus, Marco [2 ]
Al-Mestarihi, Ahmad [1 ]
Mchaourab, Hassane [2 ,3 ]
Meilerl, Jens [1 ,3 ]
机构
[1] Vanderbilt Univ, Dept Chem, Nashville, TN 37212 USA
[2] Vanderbilt Univ, Dept Mol Physiol & Biophys, Nashville, TN 37212 USA
[3] Vanderbilt Univ, Struct Biol Ctr, Nashville, TN 37212 USA
关键词
D O I
10.1016/j.str.2007.11.015
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As many key proteins evade crystallization and remain too large for nuclear magnetic resonance spectroscopy, electron paramagnetic resonance (EPR) spectroscopy combined with site-directed spin labeling offers an alternative approach for obtaining structural information. Such information must be translated into geometric restraints to be used in computer simulations. Here, distances between spin labels are converted into distance ranges between 0 carbons by using a "motion-on-a-cone" model, and a linear-correlation model links spin-label accessibility to the number of neighboring residues. This approach was tested on T4-lysozyme and alpha A-crystallin with the de novo structure prediction algorithm Rosetta. The results demonstrate the feasibility of obtaining highly accurate, atomic-detail models from EPR data by yielding 1.0 angstrom and 2.6 angstrom full-atom models, respectively. Distance restraints between amino acids far apart in sequence but close in space are most valuable for structure determination. The approach can be extended to other experimental techniques such as fluorescence spectroscopy, substituted cysteine accessibility method, or mutational studies.
引用
收藏
页码:181 / 195
页数:15
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