Interaction between cauliflower mosaic virus inclusion body protein and capsid protein: Implications for viral assembly

被引：56

作者：

Himmelbach, A ^{[1
]}

Chapdelaine, Y ^{[1
]}

Hohn, T ^{[1
]}

机构：

[1] FRIEDRICH MIESCHER INST, CH-4002 BASEL, SWITZERLAND

来源：

VIROLOGY | 1996年 / 217卷 / 01期

关键词：

D O I：

10.1006/viro.1996.0102

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The cauliflower mosaic virus (CaMV) inclusion body protein (pVI) is able to specifically interact with the viral capsid precursor protein (pIV). By using the yeast two-hybrid system and a blot assay, the pIV region required for the recognition of pVI was mapped to the lysine-rich domain. This region of only 48 amino acids when fused to dihydrofolate reductase (DHFR) mediated pVI and DNA binding in vitro. Competition experiments confirmed that pVI and DNA bind to the same region of pIV. Since pVI is absent from the mature virus, models are discussed in which pVI plays an accessory role in CaMV assembly, in addition to its function in transactivating translation. (C) 1996 Academic Press, Inc.

引用

页码：147 / 157

页数：11

共 60 条

[31]

HIMMELBACH A, 1995, THESIS U BASEL SWITZ

[32] GENETIC APPROACH TO FACILITATE PURIFICATION OF RECOMBINANT PROTEINS WITH A NOVEL METAL CHELATE ADSORBENT [J].