Mammalian homologues of the Polycomb-group gene Enhancer of zeste mediate gene silencing in Drosophila heterochromatin and at S-cerevisiae telomeres

被引:248
作者
Laible, G
Wolf, A
Dorn, R
Reuter, G
Nislow, C
Lebersorger, A
Popkin, D
Pillus, L
Jenuwein, T
机构
[1] RES INST MOL PATHOL,A-1030 VIENNA,AUSTRIA
[2] UNIV HALLE WITTENBERG,INST GENET,D-06108 HALLE,GERMANY
[3] UNIV COLORADO,DEPT MOL CELLULAR & DEV BIOL,BOULDER,CO 80309
关键词
mammalian E(z) homologues; Pc-G and PEV in Drosophila; SET-domain; TPE in S-cerevisiae;
D O I
10.1093/emboj/16.11.3219
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Gene silencing is required to stably maintain distinct patterns of gene expression during eukaryotic development and has been correlated with the induction of chromatin domains that restrict gene activity, We describe the isolation of human (EZH2) and mouse (Ezh1) homologues of the Drosophila Polycomb-group (Pc-G) gene Enhancer of zeste [E(z)], a crucial regulator of homeotic gene expression implicated in the assembly of repressive protein complexes in chromatin, Mammalian homologues of E(z) are encoded by two distinct loci in mouse and man, and the two murine Ezh genes display complementary expression profiles during mouse development, The E(z) gene family reveals a striking functional conservation in mediating gene repression in eukaryotic chromatin: extra gene copies of human EZH2 or Drosophila E(z) in transgenic flies enhance position effect variegation of the heterochromatin-associated white gene, and expression of either human EZH2 or murine Ezh1 restores gene repression in Saccharomyces cerevisiae mutants that are impaired in telomeric silencing, Together, these data provide a functional link between Pc-G-dependent gene repression and inactive chromatin domains, and indicate that silencing mechanism(s) may be broadly conserved in eukaryotes.
引用
收藏
页码:3219 / 3232
页数:14
相关论文
共 82 条
  • [61] SILENT DOMAINS ARE ASSEMBLED CONTINUOUSLY FROM THE TELOMERE AND ARE DEFINED BY PROMOTER DISTANCE AND STRENGTH, AND BY SIR3 DOSAGE
    RENAULD, H
    APARICIO, OM
    ZIERATH, PD
    BILLINGTON, BL
    CHHABLANI, SK
    GOTTSCHLING, DE
    [J]. GENES & DEVELOPMENT, 1993, 7 (7A) : 1133 - 1145
  • [62] POSITION EFFECT VARIEGATION AND CHROMATIN PROTEINS
    REUTER, G
    SPIERER, P
    [J]. BIOESSAYS, 1992, 14 (09) : 605 - 612
  • [63] DEPENDENCE OF POSITION-EFFECT VARIEGATION IN DROSOPHILA ON DOSE OF A GENE ENCODING AN UNUSUAL ZINC-FINGER PROTEIN
    REUTER, G
    GIARRE, M
    FARAH, J
    GAUSZ, J
    SPIERER, A
    SPIERER, P
    [J]. NATURE, 1990, 344 (6263) : 219 - 223
  • [64] GENETIC-TRANSFORMATION OF DROSOPHILA WITH TRANSPOSABLE ELEMENT VECTORS
    RUBIN, GM
    SPRADLING, AC
    [J]. SCIENCE, 1982, 218 (4570) : 348 - 353
  • [65] Sambrook J., 2002, MOL CLONING LAB MANU
  • [66] REDESIGNING THE BODY PLAN OF DROSOPHILA BY ECTOPIC EXPRESSION OF THE HOMEOTIC GENE ANTENNAPEDIA
    SCHNEUWLY, S
    KLEMENZ, R
    GEHRING, WJ
    [J]. NATURE, 1987, 325 (6107) : 816 - 818
  • [67] CLB5 AND CLB6, A NEW PAIR OF B-CYCLINS INVOLVED IN DNA-REPLICATION IN SACCHAROMYCES-CEREVISIAE
    SCHWOB, E
    NASMYTH, K
    [J]. GENES & DEVELOPMENT, 1993, 7 (7A) : 1160 - 1175
  • [68] ELEMENTS OF THE DROSOPHILA BITHORAX COMPLEX THAT MEDIATE REPRESSION BY POLYCOMB GROUP PRODUCTS
    SIMON, J
    CHIANG, A
    BENDER, W
    SHIMELL, MJ
    OCONNOR, M
    [J]. DEVELOPMENTAL BIOLOGY, 1993, 158 (01) : 131 - 144
  • [69] TLC1 - TEMPLATE RNA COMPONENT OF SACCHAROMYCES-CEREVISIAE TELOMERASE
    SINGER, MS
    GOTTSCHLING, DE
    [J]. SCIENCE, 1994, 266 (5184) : 404 - 409
  • [70] INHIBITION OF PLURIPOTENTIAL EMBRYONIC STEM-CELL DIFFERENTIATION BY PURIFIED POLYPEPTIDES
    SMITH, AG
    HEATH, JK
    DONALDSON, DD
    WONG, GG
    MOREAU, J
    STAHL, M
    ROGERS, D
    [J]. NATURE, 1988, 336 (6200) : 688 - 690