Combination of normal-phase medium-pressure liquid chromatography and high-performance counter-current chromatography for preparation of ginsenoside-Ro from panax ginseng with high recovery and efficiency

被引：46

作者：

Cheng, Yijun ^{[1
,2
]}

Liang, Qionglin ^{[1
]}

Hu, Ping ^{[3
]}

Wang, Yiming ^{[1
]}

Jun, Frank Wu ^{[4
]}

Luo, Guoan ^{[1
,2
]}

机构：

[1] Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China

[2] E China Univ Sci & Technol, Sch Pharm, Modern Engn Ctr Tradit Chinese Med, Shanghai 200237, Peoples R China

[3] E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

[4] XiangCam TCM Res Ctr Ltd, Unit 16, Cambridge CB22 3AT, England

来源：

SEPARATION AND PURIFICATION TECHNOLOGY | 2010年 / 73卷 / 03期

关键词：

Ginsenoside-Ro; High-performance counter-current; chromatography; Normal-phase medium-pressure liquid; Oleanane-type saponins; Panax ginseng; INDUSTRIAL SCALE-UP; DEGRADATION-PRODUCTS; DAMMARANE-SAPONINS; STATIONARY-PHASE; SEPARATION; RETENTION; PURIFICATION; NOTOGINSENG; MEYER; C.A;

D O I：

10.1016/j.seppur.2010.04.029

中图分类号：

TQ [化学工业];

学科分类号：

081705 [工业催化];

摘要：

Ginsenoside-Ro which belongs to the oleanane-type saponins has anti-inflammatory, anti-platelet, anticomplementary and immunomodulatory activities. The present paper described the preparation of ginsenoside-Ro from panax ginseng with high recovery and efficiency by combination of normal-phase medium-pressure liquid chromatography (NP-mPLC) and high-performance counter-current chromatography (HPCCC). The crude sample was preliminarily chromatographed by NP-MPLC to enrich ginsenoside-Ro to the purity of 70.2% with 95.0% recovery. Then, the enriched sample was further purified by HPCCC with a solvent system composed of ethyl acetate-isopropanol-0.1% formic acid (3:1:5, v/v), where 61 mg ginsenoside-Ro was obtained from 100 mg enriched sample with 96.0% purity and 83.4% recovery. The overall recovery of ginsenoside-Ro was 79.2%, whose structure was identified by liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) finally. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：397 / 402

页数：6

共 30 条

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