Expression of transcripts encoding AMPA receptor subunits and associated postsynaptic proteins in the macaque brain

被引:59
作者
Beneyto, M
Meador-Woodruff, JH
机构
[1] Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Psychiat, Ann Arbor, MI 48109 USA
关键词
glutamate; ionotropic receptors; postsynaptic density proteins; regulated pool; constitutive pool; in situ hybridization;
D O I
10.1002/cne.10981
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Glutamate is the primary excitatory neurotransmitter in the central nervous system, regulating numerous cellular signaling pathways and controlling the excitability of central synapses both pre- and postsynaptically. Localization, cell surface expression, and activity-dependent regulation of glutamate receptors in both neurons and glia are performed and maintained by a complex network of protein-protein interactions associated with targeting, anchoring, and spatially organizing synaptic proteins at the cell membrane. Using in situ hybridization, we examined the expression of transcripts encoding the AMPA receptor sub-units (GluR1-GluR4) and a family of AMPA-related intracellular proteins. We focused on PDZ-proteins that are involved in the regulated pool and anchoring AMPA subunits to the cell membrane (PICK1, syntenin), and those maintaining the constitutive pool of AMPA receptors at the glutamatergic synapse (NSF, stargazin). In addition, we studied a fifth protein, KIAA1719, with high homology to the rat PDZ protein ABP, associated with the clustering of AMPA receptors at the glutamate synapse. The AMPA subunits showed significant differences in regional expression, especially in the neocortex, thalamus, striatum, and cerebellum. The expression of other proteins, even those related to a specific AMPA subunit (such as ABP and PICK1 to GluR2 and GluR3), often had different distributions, whereas others (like NSF) are ubiquitously distributed in the brain. These results suggest that AMPA subunits and related intracellular proteins are differentially distributed in the macaque brain, and in numerous structures there are significant mismatches, suggesting additional functional properties of the associated intracellular proteins. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:530 / 554
页数:25
相关论文
共 103 条
  • [31] Structure and conformational changes in NSF and its membrane receptor complexes visualized by quick-freeze/deep-etch electron microscopy
    Hanson, PI
    Roth, R
    Morisaki, H
    Jahn, R
    Heuser, JE
    [J]. CELL, 1997, 90 (03) : 523 - 535
  • [32] DISASSEMBLY OF THE RECONSTITUTED SYNAPTIC VESICLE MEMBRANE-FUSION COMPLEX IN-VITRO
    HAYASHI, T
    YAMASAKI, S
    NAUENBURG, S
    BINZ, T
    NIEMANN, H
    [J]. EMBO JOURNAL, 1995, 14 (10) : 2317 - 2325
  • [33] Driving AMPA receptors into synapses by LTP and CaMKII: Requirement for GluR1 and PDZ domain interaction
    Hayashi, Y
    Shi, SH
    Esteban, JA
    Piccini, A
    Poncer, JC
    Malinow, R
    [J]. SCIENCE, 2000, 287 (5461) : 2262 - 2267
  • [34] Proteins involved in the synaptic organization of AMPA (α-amino-3-hydroxy-5-methylisoxazolepropionate) receptors
    Henley, J
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 2001, 29 : 485 - 488
  • [35] The PDZ proteins PICK1, GRIP, and syntenin bind multiple glutamate receptor subtypes - Analysis of PDZ binding motifs
    Hirbec, H
    Perestenko, O
    Nishimune, A
    Meyer, G
    Nakanishi, S
    Henley, JM
    Dev, KK
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (18) : 15221 - 15224
  • [36] Rapid and differential regulation of AMPA and kainate receptors at hippocampal mossy fibre Synapses by PICK1 and GRIP
    Hirbec, H
    Francis, JC
    Lauri, SE
    Braithwaite, SP
    Coussen, F
    Mulle, C
    Dev, KK
    Couthino, V
    Meyer, G
    Isaac, JTR
    Collingridge, GL
    Henley, JM
    [J]. NEURON, 2003, 37 (04) : 625 - 638
  • [37] CLONED GLUTAMATE RECEPTORS
    HOLLMANN, M
    HEINEMANN, S
    [J]. ANNUAL REVIEW OF NEUROSCIENCE, 1994, 17 : 31 - 108
  • [38] HUNTLEY CW, 1994, TRENDS NEUROSCI, V17, P536
  • [39] Ionotropic glutamate receptor binding and subunit mRNA expression in thalamic nuclei in schizophrenia
    Ibrahim, HM
    Hogg, AJ
    Healy, DJ
    Haroutunian, V
    Davis, KL
    Meador-Woodruff, JH
    [J]. AMERICAN JOURNAL OF PSYCHIATRY, 2000, 157 (11) : 1811 - 1823
  • [40] Nucleus-specific expression of ionotropic glutamate receptor subunit mRNAs and binding sites in primate thalamus
    Ibrahim, HM
    Healy, DJ
    Hogg, AJ
    Meador-Woodruff, JH
    [J]. MOLECULAR BRAIN RESEARCH, 2000, 79 (1-2): : 1 - 17