Expression of transcripts encoding AMPA receptor subunits and associated postsynaptic proteins in the macaque brain

被引:59
作者
Beneyto, M
Meador-Woodruff, JH
机构
[1] Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Psychiat, Ann Arbor, MI 48109 USA
关键词
glutamate; ionotropic receptors; postsynaptic density proteins; regulated pool; constitutive pool; in situ hybridization;
D O I
10.1002/cne.10981
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Glutamate is the primary excitatory neurotransmitter in the central nervous system, regulating numerous cellular signaling pathways and controlling the excitability of central synapses both pre- and postsynaptically. Localization, cell surface expression, and activity-dependent regulation of glutamate receptors in both neurons and glia are performed and maintained by a complex network of protein-protein interactions associated with targeting, anchoring, and spatially organizing synaptic proteins at the cell membrane. Using in situ hybridization, we examined the expression of transcripts encoding the AMPA receptor sub-units (GluR1-GluR4) and a family of AMPA-related intracellular proteins. We focused on PDZ-proteins that are involved in the regulated pool and anchoring AMPA subunits to the cell membrane (PICK1, syntenin), and those maintaining the constitutive pool of AMPA receptors at the glutamatergic synapse (NSF, stargazin). In addition, we studied a fifth protein, KIAA1719, with high homology to the rat PDZ protein ABP, associated with the clustering of AMPA receptors at the glutamate synapse. The AMPA subunits showed significant differences in regional expression, especially in the neocortex, thalamus, striatum, and cerebellum. The expression of other proteins, even those related to a specific AMPA subunit (such as ABP and PICK1 to GluR2 and GluR3), often had different distributions, whereas others (like NSF) are ubiquitously distributed in the brain. These results suggest that AMPA subunits and related intracellular proteins are differentially distributed in the macaque brain, and in numerous structures there are significant mismatches, suggesting additional functional properties of the associated intracellular proteins. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:530 / 554
页数:25
相关论文
共 103 条
  • [71] NSF binding to GluR2 regulates synaptic transmission
    Nishimune, A
    Isaac, JTR
    Molnar, E
    Noel, J
    Nash, SR
    Tagaya, M
    Collingridge, GL
    Nakanishi, S
    Henley, JM
    [J]. NEURON, 1998, 21 (01) : 87 - 97
  • [72] Surface expression of AMPA receptors in hippocampal neurons is regulated by an NSF-dependent mechanism
    Noel, J
    Ralph, GS
    Pickard, L
    Williams, J
    Molnar, E
    Uney, JB
    Collingridge, GL
    Henley, JM
    [J]. NEURON, 1999, 23 (02) : 365 - 376
  • [73] Ohara O, 1997, DNA Res, V4, P53, DOI 10.1093/dnares/4.1.53
  • [74] Gene expression of PSD95 in prefrontal cortex and hippocampus in schizophrenia
    Ohnuma, T
    Kato, H
    Arai, H
    Faull, RLM
    McKenna, PJ
    Emson, PC
    [J]. NEUROREPORT, 2000, 11 (14) : 3133 - 3137
  • [75] The AMPA receptor GluR2 C terminus can mediate a reversible, ATP-dependent interaction with NSF and α- and β-SNAPs
    Osten, P
    Srivastava, S
    Inman, GJ
    Vilim, FS
    Khatri, L
    Lee, LM
    States, BA
    Einheber, S
    Milner, TA
    Hanson, PI
    Ziff, EB
    [J]. NEURON, 1998, 21 (01) : 99 - 110
  • [76] Pathway-specific properties of AMPA and NMDA-mediated transmission in CA1 hippocampal pyramidal cells
    Otmakhova, NA
    Otmakhov, N
    Lisman, JE
    [J]. JOURNAL OF NEUROSCIENCE, 2002, 22 (04) : 1199 - 1207
  • [77] Paxinos G., 2000, The rhesus monkey brain in stereotaxic coordinates
  • [78] PICK1 targets activated protein kinase Cα to AMPA receptor clusters in spines of hippocampal neurons and reduces surface levels of the AMPA-type glutamate receptor subunit 2
    Perez, JL
    Khatri, L
    Chang, C
    Srivastava, S
    Osten, P
    Ziff, EB
    [J]. JOURNAL OF NEUROSCIENCE, 2001, 21 (15) : 5417 - 5428
  • [79] LIGHT AND ELECTRON IMMUNOCYTOCHEMICAL LOCALIZATION OF AMPA-SELECTIVE GLUTAMATE RECEPTORS IN THE RAT-BRAIN
    PETRALIA, RS
    WENTHOLD, RJ
    [J]. JOURNAL OF COMPARATIVE NEUROLOGY, 1992, 318 (03) : 329 - 354
  • [80] SELECTIVE RNA EDITING AND SUBUNIT ASSEMBLY OF NATIVE GLUTAMATE RECEPTORS
    PUCHALSKI, RB
    LOUIS, JC
    BROSE, N
    TRAYNELIS, SF
    EGEBJERG, J
    KUKEKOV, V
    WENTHOLD, RJ
    ROGERS, SW
    LIN, F
    MORAN, T
    MORRISON, JH
    HEINEMANN, SF
    [J]. NEURON, 1994, 13 (01) : 131 - 147