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Purification of a novel endothelin-converting enzyme specific for big endothelin-3

被引：57
作者：
Hasegawa, H
Hiki, K
Sawamura, T
Aoyama, T
Okamoto, Y
Miwa, S [1 ]
Shimohama, S
Kimura, J
Masaki, T
机构：
[1] Kyoto Univ, Fac Med, Dept Pharmacol, Sakyo Ku, Kyoto 6068315, Japan
[2] Kyoto Univ, Fac Med, Dept Neurol, Sakyo Ku, Kyoto 6068315, Japan
[3] Natl Cardiovasc Ctr, Res Inst, Suita, Osaka 5650873, Japan
来源：
FEBS LETTERS | 1998年 / 428卷 / 03期
关键词：
endothelin-3; endothelin-converting enzyme; enzyme purification; bovine iris;
D O I：
10.1016/S0014-5793(98)00554-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Endothelin-3 (ET-3), a potent vasoactive peptide, is considered to be produced from big ET-3 by endothelin-converting enzyme (ECE) like the other members of the endothelin family (ET-1 and ET-2). We purified a novel ECE from bovine iris microsomes, The purified enzyme, a 140 kDa protein by SDS-PAGE analysis, converted big ET-3 to ET-3 but not big ET-1, with a K-m value of 0.14 mu M for big ET-3. The conversion to ET-3 nas confirmed with sandwich EEA by monoclonal antibodies, the elution profile of HPLC, and intracellular calcium mobilization in CHO-K1 cells expressing recombinant human ETB receptors, The conversion activity was inhibited by an inhibitor of neutral endopeptidase 23.11 (NEP) phosphoramidon. These results show that ECE-3 purified from bovine iris is a novel metalloprotease totally different from ECE-1 or ECE-2, in that the enzyme is highly specific for big ET-3. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：304 / 308
页数：5
相关论文
共 31 条
[21]  
SAKAMOTO A, 1993, J BIOL CHEM, V268, P8547
[22]   PHOSPHORAMIDON INHIBITS THE INTRACELLULAR CONVERSION OF BIG ENDOTHELIN-1 TO ENDOTHELIN-1 IN CULTURED ENDOTHELIAL-CELLS [J].
SAWAMURA, T ;
KASUYA, Y ;
MATSUSHITA, Y ;
SUZUKI, N ;
SHINMI, O ;
KISHI, N ;
SUGITA, Y ;
YANAGISAWA, M ;
GOTO, K ;
MASAKI, T ;
KIMURA, S .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 174 (02) :779-784
[23]   MOLECULAR CHARACTERIZATION OF HUMAN AND BOVINE ENDOTHELIN-CONVERTING ENZYME (ECE-1) [J].
SCHMIDT, M ;
KROGER, B ;
JACOB, E ;
SEULBERGER, H ;
SUBKOWSKI, T ;
OTTER, R ;
MEYER, T ;
SCHMALZING, G ;
HILLEN, H .
FEBS LETTERS, 1994, 356 (2-3) :238-243
[24]   MAMMALIAN PAIRED BASIC-AMINO-ACID CONVERTASES OF PROHORMONES AND PROPROTEINS [J].
SEIDAH, NG ;
DAY, R ;
MARCINKIEWICZ, M ;
CHRETIEN, M .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1993, 680 :135-146
[25]   CLONING AND EXPRESSION OF RAT PREPROENDOTHELIN-3 CDNA [J].
SHIBA, R ;
SAKURAI, T ;
YAMADA, G ;
MORIMOTO, H ;
SAITO, A ;
MASAKI, T ;
GOTO, K .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1992, 186 (01) :588-594
[26]  
SHIMADA K, 1994, J BIOL CHEM, V269, P18275
[27]   A SENSITIVE SANDWICH-ENZYME IMMUNOASSAY FOR HUMAN ENDOTHELIN [J].
SUZUKI, N ;
MATSUMOTO, H ;
KITADA, C ;
MASAKI, T ;
FUJINO, M .
JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 118 (02) :245-250
[28]  
TAKAHASHI M, 1993, J BIOL CHEM, V268, P21394
[29]   Immunohistochemical localization of endothelin-1, endothelin-3 and endothelin receptors in human pheochromocytoma and paraganglioma [J].
Watanabe, K ;
Hiraki, H ;
Hasegawa, H ;
Tanigawa, T ;
Emura, I ;
Honma, K ;
Shibuya, H ;
Fukuda, T ;
Suzuki, T .
PATHOLOGY INTERNATIONAL, 1997, 47 (08) :540-546
[30]   ECE-1 - A MEMBRANE-BOUND METALLOPROTEASE THAT CATALYZES THE PROTEOLYTIC ACTIVATION OF BIG ENDOTHELIN-1 [J].
XU, D ;
EMOTO, N ;
GIAID, A ;
SLAUGHTER, C ;
KAW, S ;
DEWIT, D ;
YANAGISAWA, M .
CELL, 1994, 78 (03) :473-485
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