The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems

被引:41
作者
Kratasyuk, VA [1 ]
Esimbekova, EN [1 ]
Gladyshev, MI [1 ]
Khromichek, EB [1 ]
Kuznetsov, AM [1 ]
Ivanova, EA [1 ]
机构
[1] Krasnoyarsk State Agr Univ, Krasnoyarsk 660049, Russia
关键词
bioluminescence; bacterial luciferase; alcohol dehydrogenase; trypsin; water toxicity; blooming; pollution;
D O I
10.1016/S0045-6535(00)00177-6
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductaseluciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:909 / 915
页数:7
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