Glucosinolate profiling of seeds and sprouts of B-oleracea varieties used for food

被引:98
作者
Bellostas, Natalia
Kachlicki, Piotr
Sorensen, Jens C.
Sorensen, Hilmer
机构
[1] Univ Copenhagen, Fac Life Sci, Dept Nat Sci Biochem & Nat Prod Chem, DK-1871 Frederiksberg, Denmark
[2] Polish Acad Sci, Inst Plant Genet, PL-60479 Poznan, Poland
关键词
glucosinolates; sprouts; glucoraphanin; indol-3-ylmethylglucosinolates; chemoprotection;
D O I
10.1016/j.scienta.2007.06.015
中图分类号
S6 [园艺];
学科分类号
0902 ;
摘要
Consumption of plants of the species Brassica oleracea is related to a decreased incidence of certain cancer forms in humans, and this has been linked to the presence of glucosinolates in those vegetables. After ripe seeds, sprouts of some brassicaceous plants contain the highest concentration of these compounds and are therefore a good source of glucosinolates for chemoprotection. In the present experiments, the content and distribution of glucosinolates was determined in ripe seeds and sprouts (seedlings) of five varieties of B. oleracea (white cabbage, red cabbage, Savoy cabbage, broccoli and cauliflower) by high performance liquid chromatography. The type and concentration of individual glucosinolates varied according to variety of B. oleracea, plant parts in which they occurred and the sprouting period of the seed. Concentration of alkyl glucosinolates decreased whereas that of indol-3-ylmethylglucosinolates increased throughout the sprouting period. Roots had the highest glucosinolate concentration in four and seven day old sprouts whereas at both sprouting times, cotyledons had the highest concentration of alkylthio- and alkylsulphinylglucosinolates. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:234 / 242
页数:9
相关论文
共 73 条
[11]   THE GLUCOSINOLATE CONTENT OF SOME FODDER BRASSICAS [J].
BRADSHAW, JE ;
HEANEY, RK ;
SMITH, WHM ;
GOWERS, S ;
GEMMELL, DJ ;
FENWICK, GR .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1984, 35 (09) :977-981
[12]   Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana [J].
Brown, PD ;
Tokuhisa, JG ;
Reichelt, M ;
Gershenzon, J .
PHYTOCHEMISTRY, 2003, 62 (03) :471-481
[13]  
Brown PD, 1997, ADV AGRON, V61, P167, DOI 10.1016/S0065-2113(08)60664-1
[14]   Supercritical fluid chromatography as basis for identification and quantitative determination of indol-3-ylmethyl oligomers and ascorbigens [J].
Buskov, S ;
Olsen, CE ;
Sorensen, H ;
Sorensen, S .
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2000, 43 (1-3) :175-195
[15]   Determination of ascorbigens in autolysates of various Brassica species using supercritical fluid chromatography [J].
Buskov, S ;
Hansen, LB ;
Olsen, CE ;
Sorensen, JC ;
Sorensen, H ;
Sorensen, S .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (07) :2693-2701
[16]   Genetic and metabolic effects of gluconasturtiin, a glucosinolate derived from cruciferae [J].
Canistro, D ;
Della Croce, C ;
Iori, R ;
Barillari, J ;
Bronzetti, G ;
Poi, G ;
Cini, M ;
Caltavuturo, L ;
Perocco, P ;
Paolini, M .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2004, 545 (1-2) :23-35
[17]   Relationship of climate and genotype to seasonal variation in the glucosinolate-myrosinase system.: I.: Glucosinolate content in ten cultivars of Brassica oleracea grown in fall and spring seasons [J].
Charron, CS ;
Saxton, AM ;
Sams, CE .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2005, 85 (04) :671-681
[18]   Update on glucosinolate metabolism and transport [J].
Chen, S ;
Andreasson, E .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2001, 39 (09) :743-758
[19]   Content of glucosinolates in cruciferous vegetables grown at the same site for two years under different climatic conditions [J].
Ciska, E ;
Martyniak-Przybyszewska, B ;
Kozlowska, H .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (07) :2862-2867
[20]   PHYSIOLOGICAL-ROLE OF GLUCOSINOLATES IN BRASSICA-NAPUS - CONCENTRATION AND DISTRIBUTION PATTERN OF GLUCOSINOLATES AMONG PLANT ORGANS DURING A COMPLETE LIFE-CYCLE [J].
CLOSSAISBESNARD, N ;
LARHER, F .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1991, 56 (01) :25-38