RNA targets of wild-type and mutant FET family proteins

被引：273

作者：

Hoell, Jessica I. ^{[1
,2
]}

Larsson, Erik ^{[3
]}

Runge, Simon ^{[1
]}

Nusbaum, Jeffrey D. ^{[1
]}

Duggimpudi, Sujitha ^{[2
]}

Farazi, Thalia A. ^{[1
]}

Hafner, Markus ^{[1
]}

Borkhardt, Arndt ^{[2
]}

Sander, Chris ^{[3
]}

Tuschl, Thomas ^{[1
]}

机构：

[1] Rockefeller Univ, Howard Hughes Med Inst, Lab RNA Mol Biol, New York, NY 10021 USA

[2] Univ Dusseldorf, Ctr Child & Adolescent Hlth, Dept Pediat Oncol Hematol & Clin Immunol, D-40225 Dusseldorf, Germany

[3] Mem Sloan Kettering Canc Ctr, Computat Biol Ctr, New York, NY 10021 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2011年 / 18卷 / 12期

基金：

美国国家卫生研究院; 瑞典研究理事会;

关键词：

AMYOTROPHIC-LATERAL-SCLEROSIS; GENE; IDENTIFICATION; TRANSLATION; MUTATIONS; DISEASE; SITES; CLIP;

D O I：

10.1038/nsmb.2163

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

FUS, EWSR1 and TAF15, constituting the FET protein family, are abundant, highly conserved RNA-binding proteins with important roles in oncogenesis and neuronal disease, yet their RNA targets and recognition elements are unknown. Using PAR-CLIP, we defined global RNA targets for all human FET proteins and two ALS-causing human FUS mutants. FET members showed similar binding profiles, whereas FUS mutants showed a drastically altered binding pattern, consistent with changes in subcellular localization.

引用

页码：1428 / 1431

页数：4

共 15 条

[1] RNA and Disease [J].

Cooper, Thomas A. ;

Wan, Lili ;

Dreyfuss, Gideon .

CELL, 2009, 136 (04) :777-793

[2] Transcriptome-wide Identification of RNA-Binding Protein and MicroRNA Target Sites by PAR-CLIP [J].

Hafner, Markus ;

Landthaler, Markus ;

Burger, Lukas ;

Khorshid, Mohsen ;

Hausser, Jean ;

Berninger, Philipp ;

Rothballer, Andrea ;

Ascano, Manuel, Jr. ;

Jungkamp, Anna-Carina ;

Munschauer, Mathias ;

Ulrich, Alexander ;

Wardle, Greg S. ;

Dewell, Scott ;

Zavolan, Mihaela ;

Tuschl, Thomas .

CELL, 2010, 141 (01) :129-141

[3] RNA regulons: coordination of post-transcriptional events [J].

Keene, Jack D. .

NATURE REVIEWS GENETICS, 2007, 8 (07) :533-543

[4] The human genome browser at UCSC [J].

Kent, WJ ;

Sugnet, CW ;

Furey, TS ;

Roskin, KM ;

Pringle, TH ;

Zahler, AM ;

Haussler, D .

GENOME RESEARCH, 2002, 12 (06) :996-1006

[5]

Kishore S, 2011, NAT METHODS, V8, P559, DOI [10.1038/NMETH.1608, 10.1038/nmeth.1608]

[6] Mutations in the FUS/TLS Gene on Chromosome 16 Cause Familial Amyotrophic Lateral Sclerosis [J].

Kwiatkowski, T. J., Jr. ;

Bosco, D. A. ;

LeClerc, A. L. ;

Tamrazian, E. ;

Vanderburg, C. R. ;

Russ, C. ;

Davis, A. ;

Gilchrist, J. ;

Kasarskis, E. J. ;

Munsat, T. ;

Valdmanis, P. ;

Rouleau, G. A. ;

Hosler, B. A. ;

Cortelli, P. ;

de Jong, P. J. ;

Yoshinaga, Y. ;

Haines, J. L. ;

Pericak-Vance, M. A. ;

Yan, J. ;

Ticozzi, N. ;

Siddique, T. ;

McKenna-Yasek, D. ;

Sapp, P. C. ;

Horvitz, H. R. ;

Landers, J. E. ;

Brown, R. H., Jr. .

SCIENCE, 2009, 323 (5918) :1205-1208

[7] Identification of an RNA binding specificity for the potential splicing factor TLS [J].

Lerga, A ;

Hallier, M ;

Delva, L ;

Orvain, C ;

Gallais, I ;

Marie, J ;

Moreau-Gachelin, F .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (09) :6807-6816

[8] APPLICATIONS OF NEXT-GENERATION SEQUENCING RNA processing and its regulation: global insights into biological networks [J].

Licatalosi, Donny D. ;

Darnell, Robert B. .

NATURE REVIEWS GENETICS, 2010, 11 (01) :75-87

[9] mRNA Localization: Gene Expression in the Spatial Dimension [J].

Martin, Kelsey C. ;

Ephrussi, Anne .

CELL, 2009, 136 (04) :719-730

[10] Pre-mRNA Processing Reaches Back to Transcription and Ahead to Translation [J].

Moore, Melissa J. ;

Proudfoot, Nick J. .

CELL, 2009, 136 (04) :688-700

← 1 2 →