Galactose induces the expression of penicillin acylase under control of the lac promoter in recombinant Escherichia coli

被引：12

作者：

De León, A

Breceda, GB

de la Rosa, APB

Jiménez-Bremont, JF

López-Revilla, R

机构：

[1] Instr Potosino Invest Cient & Tecnol, Dept Biol Mol, San Luis Potosi 78231, Mexico

[2] Inst Tecnol Celaya, Celaya 38040, Gto, Mexico

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 17期

关键词：

expression; galactose; induction; lac promoter; penicillin acylase; pPA102; FERMENTATION CONDITIONS; FOREIGN PROTEINS; BATCH CULTURES; AMIDASE; GENE; CONSTRUCTION; INDUCTION; DYNAMICS; VECTORS; LACTOSE;

D O I：

10.1023/A:1025035019883

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The expression of penicillin acylase (PA), cloned in the pPA102 plasmid under control of the wild-type lac promoter and using galactose as inducer in Escherichia coli JM101, JM103 and JM105 transformant cells, was analyzed. The E. coli JM101/pPA102 cultures attained the highest specific activity of PA. For large scale PA production based on E. coli JM101/pPA102 a culture media with galactose instead of isopropyl-thio-galactopyranoside as inducer would be as successful and less expensive.

引用

页码：1397 / 1402

页数：6

共 24 条

[21] THE EXPRESSION OF THE PENICILLIN-G AMIDASE GENE OF ESCHERICHIA-COLI BY PRIMER EXTENSION ANALYSIS [J].