Regulation of average length of complex PCR product

被引：54

作者：

Shagin, Dmitry A. ^{[1
]}

Lukyanov, Konstantin A. ^{[1
]}

Vagner, Laura L. ^{[1
]}

Matz, Mikhail, V ^{[1
]}

机构：

[1] Inst Bioorgan Chem, Miklukho Maklaya 16-10, Moscow 117871, Russia

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 18期

关键词：

D O I：

10.1093/nar/27.18.e23

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method to achieve the preference towards longer products during PCR is described. The extent of this preference can be adjusted by slight variation of the PCR conditions. Being combined with the natural tendency of PCR to amplify shorter fragments more efficiently than longer ones, it allows one to regulate the average length of the complex PCR product over a very wide range to make it most suitable for further manipulations. The technique can be used for amplifying any complex DNA sample.

引用

页数：3

共 7 条

[1] PCR AMPLIFICATION OF UP TO 35-KB DNA WITH HIGH-FIDELITY AND HIGH-YIELD FROM LAMBDA-BACTERIOPHAGE TEMPLATES [J].

BARNES, WM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (06) :2216-2220

[2] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002

[3] Construction of cDNA libraries from small amounts of total RNA using the suppression PCR effect [J].

Lukyanov, K ;

Diatchenko, L ;

Chenchik, A ;

Nanisetti, A ;

Siebert, P ;

Usman, N ;

Matz, M ;

Lukyanov, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 230 (02) :285-288

[4] INVERTED TERMINAL REPEATS PERMIT THE AVERAGE LENGTH OF AMPLIFIED DNA FRAGMENTS TO BE REGULATED DURING PREPARATION OF CDNA LIBRARIES BY POLYMERASE CHAIN-REACTION [J].

LUKYANOV, KA ;

LAUNER, GA ;

TARABYKIN, VS ;

ZARAISKY, AG ;

LUKYANOV, SA .

ANALYTICAL BIOCHEMISTRY, 1995, 229 (02) :198-202

[5] Amplification of cDNA ends based on template-switching effect and step-out PCR [J].

Matz, M ;

Shagin, D ;

Bogdanova, E ;

Britanova, O ;

Lukyanov, S ;

Diatchenko, L ;

Chenchik, A .

NUCLEIC ACIDS RESEARCH, 1999, 27 (06) :1558-1560

[6] AN IMPROVED PCR METHOD FOR WALKING IN UNCLONED GENOMIC DNA [J].

SIEBERT, PD ;

CHENCHIK, A ;

KELLOGG, DE ;

LUKYANOV, KA ;

LUKYANOV, SA .

NUCLEIC ACIDS RESEARCH, 1995, 23 (06) :1087-1088

[7]

ZEINER M, 1994, BIOTECHNIQUES, V17, P1050

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