Multiple sites of retardation of electron transfer in Photosystem II after hydrolysis of phosphatidylglycerol

被引:25
作者
Kim, Eun-Ha [1 ]
Razeghifard, Reza [1 ]
Anderson, Jan M. [1 ]
Chow, Wah Soon [1 ]
机构
[1] Australian Natl Univ, Res Sch Biol Sci, Photobioenerget Grp, Canberra, ACT 2601, Australia
关键词
Arabidopsis thaliana; phosphatidylglycerol; phospholipase A(2); Photosystem II; thylakoid lipids; tyrosine Z;
D O I
10.1007/s11120-006-9126-0
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Phosphatidylglycerol (PG), containing the unique fatty acid Delta 3, trans-16:1- hexadecenoic acid, is a minor but ubiquitous lipid component of thylakoid membranes of chloroplasts and cyanobacteria. We investigated its role in electron transfers and structural organization of Photosystem II (PSII) by treating Arabidopsis thaliana thylakoids with phospholipase A(2) to decrease the PG content. Phospholipase A(2) treatment of thylakoids (a) inhibited electron transfer from the primary quinone acceptor Q(A) to the secondary quinone acceptor Q(B), (b) retarded electron transfer from the manganese cluster to the redox-active tyrosine Z, (c) decreased the extent of flash-induced oxidation of tyrosine Z and dark-stable tyrosine D in parallel, and (d) inhibited PSII reaction centres such that electron flow to silicomolybdate in continuous light was inhibited. In addition, phospholipase A(2) treatment of thylakoids caused the partial dissociation of (a) PSII supercomplexes into PSII dimers that do not have the complete light-harvesting complex of PSII (LHCII); (b) PSII dimers into monomers; and (c) trimers of LHCII into monomers. Thus, removal of PG by phospholipase A(2) brings about profound structural changes in PSII, leading to inhibition/retardation of electron transfer on the donor side, in the reaction centre, and on the acceptor side. Our results broaden the simple view of the predominant effect being on the Q(B)-binding site.
引用
收藏
页码:149 / 158
页数:10
相关论文
共 37 条
[1]   Unifying model for the photoinactivation of Photosystem II in vivo under steady-state photosynthesis [J].
Anderson, JM ;
Park, YI ;
Soon, WS .
PHOTOSYNTHESIS RESEARCH, 1998, 56 (01) :1-13
[2]   DICHLOROPHENYLUREA-INSENSITIVE REDUCTION OF SILICOMOLYBDIC ACID BY CHLOROPLAST PHOTOSYSTEM-II [J].
BARR, R ;
CRANE, FL ;
GIAQUINTA, RT .
PLANT PHYSIOLOGY, 1975, 55 (03) :460-462
[3]   THE SULFOLIPID SULFOQUINOVOSYLDIACYLGLYCEROL IS NOT REQUIRED FOR PHOTOSYNTHETIC ELECTRON-TRANSPORT IN RHODOBACTER-SPHAEROIDES BUT ENHANCES GROWTH UNDER PHOSPHATE LIMITATION [J].
BENNING, C ;
BEATTY, JT ;
PRINCE, RC ;
SOMERVILLE, CR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (04) :1561-1565
[4]   Electron fluxes through Photosystem I in cucumber leaf discs probed by far-red light [J].
Chow, WS ;
Hope, AB .
PHOTOSYNTHESIS RESEARCH, 2004, 81 (01) :77-89
[5]   DIRECTED MUTAGENESIS INDICATES THAT THE DONOR TO P-680+ IN PHOTOSYSTEM-II IS TYROSINE-161 OF THE D1 POLYPEPTIDE [J].
DEBUS, RJ ;
BARRY, BA ;
SITHOLE, I ;
BABCOCK, GT ;
MCINTOSH, L .
BIOCHEMISTRY, 1988, 27 (26) :9071-9074
[6]   Phosphatidylglycerol is essential for oligomerization of photosystem I reaction center [J].
Domonkos, I ;
Malec, P ;
Sallai, A ;
Kovács, L ;
Itoh, K ;
Shen, GZ ;
Ughy, B ;
Bogos, B ;
Sakurai, I ;
Kis, M ;
Strzalka, K ;
Wada, H ;
Itoh, S ;
Farkas, T ;
Gombos, Z .
PLANT PHYSIOLOGY, 2004, 134 (04) :1471-1478
[7]   BIOCHEMISTRY AND FUNCTION OF THE PLASTID ENVELOPE [J].
DOUCE, R ;
JOYARD, J .
ANNUAL REVIEW OF CELL BIOLOGY, 1990, 6 :173-216
[8]   The role of phospholipids in regulating photosynthetic electron transport activities: Treatment of thylakoids with phospholipase C [J].
Droppa, M ;
Horvath, G ;
Hideg, E ;
Farkas, T .
PHOTOSYNTHESIS RESEARCH, 1995, 46 (1-2) :287-293
[9]   The topology of phosphatidylglycerol populations is essential for sustaining photosynthetic electron flow activities in thylakoid membranes [J].
Duchêne, S ;
Smutny, J ;
Siegenthaler, PA .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1463 (01) :115-120
[10]   Phosphatidylglycerol requirement for the function of electron acceptor plastoquinone QB in the photosystem II reaction center [J].
Gombos, Z ;
Várkonyi, Z ;
Hagio, M ;
Iwaki, M ;
Kovács, L ;
Masamoto, K ;
Itoh, S ;
Wada, H .
BIOCHEMISTRY, 2002, 41 (11) :3796-3802