Selectivity enhancement for glutamate with a Nafion/glutamate oxidase biosensor

被引：67

作者：

Pan, ST ^{[1
]}

Arnold, MA ^{[1
]}

机构：

[1] UNIV IOWA, DEPT CHEM, IOWA CITY, IA 52242 USA

来源：

TALANTA | 1996年 / 43卷 / 07期

基金：

美国国家科学基金会;

关键词：

glutamate; Nafion/glutamate oxidase biosensor; selectivity enhancement;

D O I：

10.1016/0039-9140(95)01854-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Response properties and selectivity are reported for glutamate biosensors constructed with a film of Nafion between the platinum anode and a layer of immobilized glutamate oxidase. The effects of enzyme loading, sample pH and temperature are established. Operation at pH 7.8 and 37 degrees C results in linearity up to 800 mu M and a limit of detection of 0.3 mu M. Nafion enhances selectivity for glutamate over test species that include ascorbic acid, uric acid and acetaminophen. Selectivity enhancement was greater over the anionic interferences because of electrostatic repulsion and the extent of this enhancement depends on the thickness of the Nafion layer. Even under ideal conditions, some interfering signal is observed when glutamate levels are ten-times less than ascorbate.

引用

页码：1157 / 1162

页数：6

共 15 条

[11]

Trevan MD, 1980, IMMOBILIZED ENZYMES

[12] DUAL-ENZYME FIBEROPTIC BIOSENSOR FOR GLUTAMATE BASED ON REDUCED NICOTINAMIDE ADENINE-DINUCLEOTIDE LUMINESCENCE [J].