Twelve-hour PCR-based method for detection of Salmonella spp. in food

被引:98
作者
Ferretti, R
Mannazzu, I
Cocolin, L
Comi, G
Clementi, F
机构
[1] Dipartimento Biotechnol Agr & Ambientali, I-60100 Ancona, Italy
[2] Dipartimento Sci Alimenti, I-33100 Udine, Italy
关键词
D O I
10.1128/AEM.67.2.977-978.2001
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A PCR-based method for the detection of Salmonella spp. in food was developed. The method, set up on typical salami from the Italian region of Marche, is sensitive and specific and shows excellent correlation with the conventional method of reference when naturally contaminated foods are analyzed. Moreover, it can be easily performed within a maximum of 12 h from food sampling, thus allowing prompt detection of Salmonella spp. in the food stocks analyzed.
引用
收藏
页码:977 / 978
页数:2
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