Population structure and characterization of viridans group streptococci (VGS) including Streptococcus pneumoniae isolated from adult patients with cystic fibrosis (CF)

被引：46

作者：

Maeda, Yasunori ^{[1
,2
]}

Elborn, J. Stuart ^{[3
,4
]}

Parkins, Michael D. ^{[4
,5
,6
]}

Reihill, James ^{[3
]}

Goldsmith, Colin E. ^{[1
]}

Coulter, Wilson A. ^{[7
]}

Mason, Charlene ^{[7
]}

Millar, B. Cherie ^{[1
]}

Dooley, James S. G.

Lowery, Colm J. ^{[2
]}

Ennis, Madeleine ^{[3
]}

Rendall, Jacqueline C.

Moore, John E. ^{[1
,2
]}

机构：

[1] Belfast City Hosp, No Ireland Publ Hlth Lab, Dept Bacteriol, Belfast BT9 7AD, Antrim, North Ireland

[2] Univ Ulster, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland

[3] Queens Univ Belfast, Ctr Infect & Immun, Dept Resp Med, Belfast City Hosp, Belfast BT9 7AB, Antrim, North Ireland

[4] Belfast City Hosp, No Ireland Reg Adult Cyst Fibrosis Unit, Belfast BT9 7AB, Antrim, North Ireland

[5] Univ Calgary, Dept Med, Calgary, AB, Canada

[6] Calgary Hlth Reg, Calgary, AB, Canada

[7] Queens Univ Belfast, Sch Dent, Royal Grp Hosp, Belfast, Antrim, North Ireland

来源：

JOURNAL OF CYSTIC FIBROSIS | 2011年 / 10卷 / 02期

关键词：

CELL-WALL POLYSACCHARIDES; PSEUDOMONAS-AERUGINOSA; DENTAL PLAQUE; COAGGREGATION; VEILLONELLA; ACTINOMYCES; INFECTIONS;

D O I：

10.1016/j.jcf.2010.11.003

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

A study was undertaken to examine the population structure of viridans group streptococci (VGS) in the sputum of adult patients with cystic fibrosis (CF). Freshly expectorated sputa (n=58) from 45 adult CF patients were examined by selective conventional culture on Mitis-Salivarius agar and yielded 190 isolates of VGS. Sequence analyses of the rpnB and 16-23S rRNA ITS genes identified these isolates to belong to 12 species of VGS and included S. anginosus, S. australis, S. cristatus, S. gordonii, S. infantis, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. pneumoniae, S. salivarius and S. sanguinis. The most frequently VGS organism isolated was S. salivarius (47/190; 24.7%), followed by S. mitts (36/190; 19%), S. sanguinis (25/190; 13.2%), S. oralis (20/190; 11.0%), S. pneumoniae (19/190; 10.0%), S. parasanguinis (16/190; 8.4%), S. infantis (11/190; 5.8%), S. gordonii (7/190; 3.7%), S. anginosus (4/190; 2.1%), S. cristatus (2/190; 1.1%), S. australis (1/190; 0.5%), S. mutans (1/190; 0.5%) and S. agalactiae (1/190; 0.5%). All, but four, patients harboured at least one VGS species, which ranged from one to five streptococcal species, with a mean of 2.85 species per patient. There was no clonality at the subspecies level employing ERIC RAPD PCR. Antibiotic susceptibility was determined by Minimum Inhibitory Concentration (MIC) testing against penicillin, erythromycin and ciprofloxacin. Overall, resistance to penicillin with all VGS was 73/190 (38.4%) and 167/190 (87.9%) for erythromycin. With regard to ciprofloxacin, 27/190 (14.2%) were fully resistant, whilst a further 21/190 (11.1%) showed intermediate resistance, which equated to approximately three quarters (74.7%) of isolates being fully sensitive to this agent. In addition, as a comparator control population, we examined antibiotic susceptibility, as above, in a non-CF population comprising 12 individuals (50 VGS isolates), who were not receiving chronic antibiotics. In comparison, 8% and 38% of VGS isolates from non-CF individuals were resistant by disk susceptibility testing to penicillin and erythromycin, respectively. None of the non-CF VGS organisms were resistant to ciprofloxacin, but 42% showed intermediate resistance. (C) 2010 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

引用

页码：133 / 139

页数：7

共 21 条

[1] Characterization of a Streptococcus sp.-Veillonella sp Community Micromanipulated from Dental Plaque [J].