Reversed-phase high-performance liquid chromatographic screening method for serum steroids using retention index and diode-array detection

被引：16

作者：

Kuronen, P ^{[1
]}

Volin, P ^{[1
]}

Laitalainen, T ^{[1
]}

机构：

[1] Univ Helsinki, Dept Chem, Organ Chem Lab, FIN-00014 Helsinki, Finland

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 718卷 / 02期

关键词：

steroids;

D O I：

10.1016/S0378-4347(98)00351-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A multisteroid screening method has been developed based on the use of 1-[4-(2,3-dihydroxypropoxy)phenyl]-1-alkanones as retention index standards and UV absorbance spectra recorded on-line with a diode-array detector using reversed-phase high-performance liquid chromatographic gradient elution with acetonitrile and water. The effect of chromatographic conditions on retention indices of steroids were studied. The method was tentatively applied to profiling of steroids in serum samples. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：211 / 224

页数：14

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