HSCCC-MS study of flavonoids in the extracts from the seeds of Oroxylum indicum

被引：16

作者：

Chen, LJ

Song, H

Games, DE

Sutherland, IA

机构：

[1] Sichuan Univ, W China Univ Med Sci, W China Hosp, State Key Lab Biotherapy & Canc Ctr, Chengdu 610041, Peoples R China

[2] Sichuan Univ, Dept Pharmaceut & Bioengn, Chengdu, Peoples R China

[3] Univ Coll Swansea, Mass Spectrometry Res Unit, Swansea, W Glam, Wales

[4] Brunel Univ, Brunel Inst Bioengn, Uxbridge UB8 3PH, Middx, England

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2005年 / 28卷 / 12-13期

关键词：

HSCCC; flavonoids; Oroxylum indicum;

D O I：

10.1081/JLC-200063651

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, two analytical high-speed countercurrent chromatography (HSCCC) instruments, interfaced directly with electrospray ionization and atmospheric pressure chemical ionization mass spectrometry, were explored for the separation of a mixture of three standard flavonoids. Hexane-ethyl acetate-methanol-water solvent systems with different ratios were chosen and optimized for the best separation. On-line HSCCC-MS was successfully used in the identification of flavonoids from an ethyl acetate extract of the seeds of Oroxylum indicium.

引用

页码：1993 / 2003

页数：11

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