Protective efficacy of recombinant urease B and aluminum hydroxide against Helicobacter pylori infection in a mouse model

被引：17

作者：

Begue, Rodolfo E. ^{[1
]}

Sadowska-Krowicka, Halina ^{[2
]}

机构：

[1] Louisiana State Univ, Hlth Sci Ctr, Dept Pediat, Div Infect Dis,Childrens Hosp, New Orleans, LA 70118 USA

[2] Louisiana State Univ, Hlth Sci Ctr, Dept Pediat, Div Res,Childrens Hosp, New Orleans, LA 70118 USA

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2010年 / 60卷 / 02期

关键词：

adjuvant; bacterial vaccine; Helicobacter pylori; mouse model; urease B; REAL-TIME PCR; ORAL IMMUNIZATION; IMMUNE-RESPONSES; MICE; FELIS; VACCINE; IMMUNOGENICITY; GASTRITIS; ADJUVANT; SAFETY;

D O I：

10.1111/j.1574-695X.2010.00726.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Efforts are underway for the development of an effective vaccine against Helicobacter pylori infection. We prepared recombinant full-length (568 aa) H. pylori recombinant urease B (rUreB) protein and tested it for immunogenicity and protection. BALB/c mice received either rUreB (40 mu g) plus CpG (10 mu g) intranasally, rUreB (50 mu g) plus 3% aluminum hydroxide (50 mu L) intramuscularly or rUreB (25 mu g) plus Freund's adjuvant (25 mu L) subcutaneously, three times (weeks 0, 2 and 6). Intranasal rUreB plus CpG was neither immunogenic nor protective; intramuscular rUreB plus aluminum hydroxide was immunogenic and modestly protective, and subcutaneous rUreB plus Freund's adjuvant was immunogenic and highly protective. The fact that protection was improved with Freund's adjuvant indicates that rUreB is a good antigen for a vaccine but that it needs a stronger adjuvant than aluminum hydroxide.

引用

页码：142 / 146

页数：5

共 27 条

[1] Immunogenicity of Helicobacter pylori urease B protein and DNA vaccines in a mouse model [J].